Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,134	G→A	G250S (GGT→AGT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,134	0	G	A	100.0%	17.9 / NA	8	G250S (GGT→AGT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

AACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGAC  >  NC_000913/70888‑71341                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                aaCGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAACgtcagtc                                                                                                                                                                                                              <  1:218062/250‑1 (MQ=255)                                                                                                         gCATGTTTGCTTGTCGCCGTCGTGTCTGGCACATCTTATCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGCCCAACGCATTAGTCAGACGAAGCTGCTTTTGAGCACTTCACGGATGTCTAGCGCTCCCGACAGTGGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCCCCGGGGCCGGCCCGAGCGAGGTTCGTGCCGGTTATGAAGAAAAAGGGAatg                                                                                                     <  1:9741/251‑1 (MQ=255)                                                                                                                                                                                                 aTTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCttn             <  1:55800/251‑2 (MQ=255)                                                                                                                                                                                                    aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATGGGGTTTGCAGAATCCCTGCTTCGTc          >  1:2529/1‑251 (MQ=255)                                                                                                                                                                                                     gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGATAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTcc         >  1:212202/1‑251 (MQ=255)                                                                                                                                                                                                             caGCTGCTTTTGAGCACTACCCGGATGCCTCTCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGAc  >  1:60120/2‑250 (MQ=255)                                                                                                                                                                                                                   ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCCACTCTATTTCTAGCGCGTATTTAAAAAATGACCCGGGGCCAGTCCGAGCGAGTTTCGTGCCGGATGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATACTTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTc          >  1:114769/1‑236 (MQ=255)                                                                                                                                                                                                                                        ccTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAAGCAGACAATTGAc                                                      <  1:166615/171‑1 (MQ=255)                                                                                                                                                                                                                                              gCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTATCGCGAGTATTTACAACATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGACGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGCCGGCTTGACGGAGTAGCATAGGGTTTGCAGAAt                      >  1:18125/1‑197 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                AACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGAC  >  NC_000913/70888‑71341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑