Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,705	C→T	A171A (GCG→GCA)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,705	0	C	T	100.0%	31.4 / NA	12	A171A (GCG→GCA)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

TACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATC  >  NC_000913/667492‑667948                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    tACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTGAACTTCATCCTCTTCATAACCGGGCTGTCCGTGCAGCACATCTTCAGAGTAACGCTCAATGGCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTGTGCCGTCATTATTCAGGGTTTCTACGTCTTGGTCGGTGATTTTCGACACATAGCCTATTACTTGGGTCAATTCCGAACCGTAAGGATAGTAACGACGTTTATAGCCt                                                                                                                                                                                                                 <  1:143494/250‑1 (MQ=255)                          ngCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGCCCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATCGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAAc                                                                                                                                                                                                            >  1:38610/2‑230 (MQ=255)                                    tgttAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGan                                                                                                                                                                              <  1:129519/250‑2 (MQ=255)                                            tCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCg                                                                                                                                                                     >  1:117097/1‑251 (MQ=255)                                                                       tGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCCTCATTATTCAGGCGTTCGAAGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCCATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGt                                                                                                                                          >  1:102977/1‑251 (MQ=255)                                                                           gcaCATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGc                                                                                                                                                                           >  1:50554/1‑214 (MQ=255)                                                                                            cGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGc                                                                                                                                                                      >  1:15225/1‑202 (MQ=255)                                                                                                                        gCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGGCGGCAAAGCTAGGTACTTGTACTTCGGGCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCgg                                                                                         <  1:205323/251‑1 (MQ=255)                                                                                                                                                                      gacgtttgtATCGTTGATTTTCGACACATAGCCGATGACGTGGGTTAATGCCGAACCGGAAGGATAGTAACGACGTTGAGAGCCTTTAACTTCGACACCCGGAAAGCGGGACTTGTTGACGGTCAAGCGAGTTACTTGTGCTTCGGTCATGGTAGTTTTCACCGGAAGAGAGGTTAAACGGTTTGATCGTGCGCTCTCTTTTCGGAATGCAGCTATATCGTCATCGGTCAGATCTACCACGCTGCGCaaan                                           <  1:23976/243‑2 (MQ=255)                                                                                                                                                                           cTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTGTAACTTCGACACCCGGAAAACGGTACTGATTGCCGGCAAAGCGAGCTACGTGGACTTCGGTCAGGTTAGTTTTCACCGGAATAGAgg                                                                                                                         <  1:196880/168‑1 (MQ=255)                                                                                                                                                                                                   aNNCGATGANNTGGGTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGc                                                                                                                                                                      <  2:15225/99‑1 (MQ=255)                                                                                                                                                                                                                ggTCAATGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGcatc  >  1:100607/1‑250 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATC  >  NC_000913/667492‑667948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑