Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,586	C→T	G202D (GGT→GAT)	rlpA ←	rare lipoprotein RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,586	0	C	T	100.0%	24.1 / NA	10	G202D (GGT→GAT)	rlpA	rare lipoprotein RlpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

GTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACC  >  NC_000913/664355‑664798                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      gtCACAATTGCAGGCGAGGATGCAGGCGTTGTCGACGGAGCTGTAACAACGGTCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCGGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGATTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTgc                                                                                                                                                                                                    >  1:45535/1‑251 (MQ=255)                                    cGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGACGACTGTGGTAc                                                                                                                                                                 >  1:181872/1‑251 (MQ=255)                                                                                           ccAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGTCAGACACTGAACTTGTTCCCGCGCCATCGCTTACATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTgg                                                                                                                                                                             >  1:124441/1‑184 (MQ=255)                                                                                              ngcgcTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTAGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGAGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCCGACACTGAAATTGTTCACGCGCCATCGCTTACAACGGGAGGTGCAGACAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGACACC‑TCCTAGGCGACAATAATCGGATCGaaccg                                                                                                      >  1:44812/2‑247 (MQ=255)                                                                                                                     gAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACgtgt                                                                               >  1:113675/1‑251 (MQ=255)                                                                                                                             tctgaCTGGTGACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTGGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACg                                                                        <  1:34020/246‑1 (MQ=255)                                                                                                                                tACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCCCCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACg                                                                        <  1:34024/247‑1 (MQ=255)                                                                                                                                                                         gTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGAAACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGCACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGcc                              >  1:90908/1‑248 (MQ=255)                                                                                                                                                                                                  cACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGCGGTGCAGGCAGGGCGTCAGTCTGTTTGGCGACTGTGGTACCAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATACTCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCa                                                                    >  1:102653/1‑185 (MQ=255)                                                                                                                                                                                                   aCCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCATCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGcacc  >  1:46285/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      GTCACCATTGCAGGCGAGGTTGCAGGCGTTGTCGACGGAGCTGTAACAACGGGCTGTGGCGCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGA‑ACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACC  >  NC_000913/664355‑664798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑