Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,146,163	G→A	R723R (CGG→CGA)	pflD →	putative formate acetyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,163	0	G	A	100.0%	18.1 / NA	10	R723R (CGG→CGA)	pflD	putative formate acetyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

GACGGCGGCTTGTCACCCATGCTGGGCCAGGACGCACAAGGGCCAACAGCGGTACTGAAGTCAGTCAGTAAGCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTT‑AAAGGCTGTCCGCATCT  >  NC_000913/4145924‑4146406                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gACGGCGGCTTGTCACCCATGCTGGGCCAGGACGCACAAGGGCCAACAGCGGTAATGAAGTCAGTCAGTACGCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCAATCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGACGACACGTTGCGAGAAGCGCAAc                                                                                                                                                                                                                                            >  1:91480/1‑250 (MQ=255)                                                    gTACTGAAGTCAGTAAGTAAGCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGAT‑ATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGcc                                                                                                                                                                                       >  1:70809/1‑251 (MQ=255)                                                                    ntAAGCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTg                                                                                                                                                                        >  1:52698/2‑251 (MQ=255)                                                                                                                   aaaTTCACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCNACANNNNNcann                                                                                                                                                                                                                                  >  2:127733/1‑144 (MQ=255)                                                                                                                        cACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCg                                                                                                                                                                             <  1:63706/194‑1 (MQ=255)                                                                                                                                         aaGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTAAGTGAGTTTACCCAGCATGAGTTACAACATATTCAGTTTATAGTGGTGACCGCCGACCCGTTGCGAGAAGCGCATAAGCGCCCACACGATTCTGACGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACAGTAGAAGGAGATCAAGGATGACATCATCCGACGGATAGCGCATCAGCTGTACCGTTGAGGAAACGcgccg                                                                                                   >  1:27876/1‑251 (MQ=255)                                                                                                                                            gTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGAGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTCGCCGGATACa                                                                                                                                                                                            >  1:216638/1‑159 (MQ=255)                                                                                                                                                                                             gcgtAAGTTACAACAGATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAgcg                                                                                                                                                                                         <  1:79991/110‑1 (MQ=255)                                                                                                                                                                                                             atTCAGTTTAACGTGGTGAACGCCGACACGTTGCGAGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACgg                               <  1:109425/251‑1 (MQ=255)                                                                                                                                                                                                                                          gTTGCGAGAAGCGACACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCAGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATAAGCTGTAACGTTGTGGAACCGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCt  >  1:157311/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GACGGCGGCTTGTCACCCATGCTGGGCCAGGACGCACAAGGGCCAACAGCGGTACTGAAGTCAGTCAGTAAGCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCAGGATTACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTACAACATATTCAGTTTAACGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTT‑AAAGGCTGTCCGCATCT  >  NC_000913/4145924‑4146406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑