Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,832,474	G→A	F688F (TTC→TTT)	yicI ←	alpha‑D‑xyloside xylohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,832,474	0	G	A	100.0%	36.5 / NA	14	F688F (TTC→TTT)	yicI	alpha‑D‑xyloside xylohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

CCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACC  >  NC_000913/3832281‑3832684                                                                                                                                                                                                  |                                                                                                                                                                                                                   ccTGCGAACCGTCTTGCAGACCATTTACTTTAACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCACGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGcc                                                                                                                                                           >  1:185122/1‑251 (MQ=255)                 cAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCAc                                                                                                                                           <  1:17163/251‑1 (MQ=255)                                 acaacaTTGCGCAGGCACAGTGTCCAGTTCTCCGCCTCGCCCGCACCAGTCACATTAAGCGTGTTGCCAGTAGGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGTGCACTTCACAGACTGCTTCATGCCCGGCTGGCAGTTTAAAGTTATGAAATCCCGTGCCTTCTGGCCACACGTAATCGTGACCATGAGTGTTGTTGCCCTGCGCCATTAGAGTGTTATCACGCACATAAACGGGCAg                                                                                                                           <  1:200129/251‑1 (MQ=255)                                         gcgcAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGGAAAGATCACCGATCCGTCAGCAGCGGGCTCTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCGCACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAgg                                                                                                                    <  1:109685/250‑1 (MQ=255)                                               gCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTGCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCTCACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCTCATAAACGGGCAGACTCAGGAAGCCGt                                                                                                             <  1:65899/251‑1 (MQ=255)                                                                        cccGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAACGATCACCGATCCGTCAGCAGCGGGCCCTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCCGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTAcc                                                                                    >  1:74221/1‑251 (MQ=255)                                                                            cACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCg                                                                                <  1:31688/251‑1 (MQ=255)                                                                                                   ccAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGc                                                                                               <  1:70066/213‑1 (MQ=255)                                                                                                                       agtaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGCCGGCTTCTTGACCGCCTTCCAGATTGCAGCAGCGATATTCCGCCCCTTCGTGCCCTAGGTGATCGGGCCGTTGATCGTTGTTGCCCCGCGGCCGTAGAGTGTTACCATGCGCATAACCGGGCAGTCTCAGACAGCCGTACTGATGTTTATGCCGGCGACTACCGCCGAGGTCATCGTTGGGCCACAGGGGTGGCCAGCGACCTTCCGGc                                      >  1:216515/1‑250 (MQ=255)                                                                                                                        ntaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGACCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGc                                      >  1:39550/2‑249 (MQ=255)                                                                                                                                                     cACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGcn                                                                                              <  1:54268/164‑2 (MQ=255)                                                                                                                                                          nacaGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATAACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACAcc  >  1:24765/2‑251 (MQ=255)                                                                                                                                                                        tGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTNCTGNNNNNGcnnnnnn                                                                                         >  2:133274/1‑144 (MQ=255)                                                                                                                                                                        tGCCCGTCTTGCAGATTGAAGAGGTGAAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTtgtn                                                                  <  1:133274/173‑2 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                   CCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACC  >  NC_000913/3832281‑3832684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑