Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	38.6 / NA	14	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/7);  total (7/7)

CAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTC  >  NC_000913/3485989‑3486441                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             cAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCACCTTCCCAGGCAGCGGGAAGCATATTTAGGCAATCCAGCGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                                                                                                                                                                                            >  1:189542/1‑251 (MQ=255)        cGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCAAGGCAGCGGGAAGCATATTTCGGCACTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAGGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGCTCTTGTCTCATTGCCAAATTCATAAGTACCCATCCAAGAGCAAGCTTCTTATCCAGGGTGAAAAAGCGGAAACGCTGtactac                                                                                                                                                                                                      >  1:191569/1‑250 (MQ=255)                                     gCCCCTTCCCGGAGAGCGGGAAGCATNTTTCGGNNATNCAGAGACAGCGGCGTTATCNGGNNNTNNNGNAAGCTNATNNCAGAGGATANCCGCGCATGGAGCTTG‑GCAAACCGCAAACAGACCCGACTCTAGAATGGTTCTTNTCTCATTGCNNCATTCATAAGTACCCATCCAANAGCNNGNNTNTTcntc                                                                                                                                                                                                                                   >  2:50563/1‑189 (MQ=255)                                      ccccTGCCCAGGTAGCGGGAATCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGTGCATGGTG‑TTGTGCAAACCGCAATAATTCCCGACTCTCGAATGGTTCTGTTCTCATTGCCACATTCAGATGTTCCCAGCCAGGAACAACCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCa                                                                                                                                                                                 <  1:119377/241‑1 (MQ=255)                                                            natatTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAAt                                                                                                                                                 >  1:55541/2‑251 (MQ=255)                                                                                   gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCCTGGTGCTTG‑GCAAACCGCCAACAGACCCGACTCTCGACTGGTTCTTGTCTCATTGCCACATTCCTAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAAGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGGAAAGAAATGATCCTCTCCTATCTGAATCAgg                                                                                                                          >  1:124175/1‑251 (MQ=255)                                                                                                        gaAAGCTTNTNNNANNGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACNCTCGAATGGTTCTTGNCTNATTGNCACNTTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                                                                                                        >  2:148918/1‑184 (MQ=255)                                                                                                        gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                                                                                                        <  1:148918/184‑1 (MQ=255)                                                                                                                    cAGAGGATACCCGCGCATGGGGCTTG‑GCAATCCGCAAACAGCCCCGACACTCGAATGGTTCTTGTCTCATTTCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAATGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGTGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag                                                                                         <  1:31855/251‑1 (MQ=255)                                                                                                                                    aTGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAGTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCGGTGGCAGTGCGGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTan                                                                         <  1:19882/251‑2 (MQ=255)                                                                                                                                           ttG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCTGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                                                                                            <  1:118035/145‑1 (MQ=255)                                                                                                                                                           cAGACCCGACTCTCGAATGGTTCTTGTCTCAGTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGGTGATCAAAGACGAAGAGGGGAAAGAAATGATCCTCTCCTAGCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAAcc                                                   <  1:160427/251‑1 (MQ=255)                                                                                                                                                                  gACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAATGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAc                                                                                                        <  1:113751/191‑1 (MQ=255)                                                                                                                                                                                                            nccATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTCCATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGACATGATCCTCTCCTATCTGAATCAgg                                                                                                                          >  1:23013/2‑131 (MQ=255)                                                                                                                                                                                                            cccATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc  >  1:190541/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             CAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTG‑GCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTC  >  NC_000913/3485989‑3486441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑