Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,404,851	G→A	intergenic (+393/‑585)	yhdH → / → accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,851	0	G	A	100.0%	11.4 / NA	7	intergenic (+393/‑585)	yhdH/accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTTCGTGAATTGTGCGGCTTGTTGCAAATTACACGGTGTTGAAG  >  NC_000913/3404617‑3405084                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          caAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCAGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAAACGATGc                                                                                                                                                                                                                                    >  1:123383/1‑242 (MQ=255)                 gATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTACTTAGCGTGAGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGCGAATAATCTGATTTTGTTTGACTACAAATTAATAACTCGAACCTATTTAATGCTGAGCATTGTCACTCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAGc                                                                                                                                                                                                            >  1:96566/1‑250 (MQ=255)                                  cTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTCGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGCGAATAATCTGATTTTGTTTGAATACAAATTAATCAATCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAg                                                                                                                                                                                                             >  1:188142/1‑232 (MQ=255)                                              cGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAACTTTGCGTGCTTTAGCATTCACATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcg                                                                                                                                                                              <  1:123866/251‑1 (MQ=255)                                                       cGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTgg                                                                                                                                                                     <  1:171659/251‑1 (MQ=255)                                                                                                                                                                                                                     cTTTAGCATTCAAATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGACGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTATGGTCTGTGCAGATTATCTCATTGCACCCACTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTTCGTGACTTGTGCGGCTTGTTGCAAATTACACGgtgt       >  1:115113/1‑251 (MQ=255)                                                                                                                                                                                                                          gCATTCACATCTATCCAAACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTTCGTGAATTGTGCGGCTTGTTGCAAATTACACGGTGTTGAAg  <  1:86770/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          CAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTTCGTGAATTGTGCGGCTTGTTGCAAATTACACGGTGTTGAAG  >  NC_000913/3404617‑3405084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑