Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,086	G→A	W189* (TGG→TGA)	aaeR →	DNA‑binding transcriptional activator AaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,086	0	G	A	100.0%	12.2 / NA	6	W189* (TGG→TGA)	aaeR	DNA‑binding transcriptional activator AaeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

GCCAAAATGCTGAAAGAATACCCAGGTTTGAGCGTCAATCTGGTTACCGGAATTCCAGCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCG‑CGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGA  >  NC_000913/3389853‑3390261                                                                                                                                                                                                                                           |                                                                                                                                                                                cccAAAATCCTAAAAGAATCCCCAGGTTTGAGCGTCAATCTGGTTACCGGAATTCCCGCCCCCGACCTGATTGCCTACGTTCTGGATTTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCCCCGTCTGGCCGCGATGCCAATGGTGGTGTGCGCCG‑CAAAAATCTACCTCACACAATCCGGCACACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCg                                                                                                                                                                <  1:147790/250‑1 (MQ=255)                                gCGTCAATCTGGTTACCGGAATTCCAGCCCCCGACCTGCTTGCCGACGGTATGGCTGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCG‑CGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCg                                                                                                                                 >  1:89882/1‑251 (MQ=255)                                                                                                      nggCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCG‑CGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCAGATCCCACAAGGAAGCTTTGTGACTAATGATCCGATGACGCTGGTGCgct                                                           >  1:24832/2‑251 (MQ=255)                                                                                                       ggCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGGGGGCGCCG‑CGAAAAGCTATCTCACACAAAACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctn                                                          <  1:128823/251‑2 (MQ=255)                                                                                                                         cgCCTGTTTTCCCGCCGTCTGGGCGCTAGGCTAATGGTTGGGTG‑TCCGACGAAAAGTTATCCCACCCATTACGGAAAACCGGAAAATCCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAATGAAGATTTTTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCgg                                        <  1:88667/250‑1 (MQ=255)                                                                                                                                                               ggtgTGCGCCG‑CAAAAAGCTATCCCCCCCAATCCGACAACCCGGAAAACCCCGCCGATTTGAGTAGTCATTCATGACTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGTGATCGCCTACGTGCCGCTGATGTGGGTGATCAACgaga  <  1:74356/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                GCCAAAATGCTGAAAGAATACCCAGGTTTGAGCGTCAATCTGGTTACCGGAATTCCAGCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCG‑CGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGA  >  NC_000913/3389853‑3390261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑