Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	525,335	G→A	S692N (AGT→AAT)	rhsD →	protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	525,335	0	G	A	100.0%	27.0 / NA	12	S692N (AGT→AAT)	rhsD	protein RhsD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)

CCGGACGGCAACCGGAGCGAGACACAGTACGATGCGTGGGGAAAGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAGTGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTGACCGGCGAATGCCAGACGGTGGAGAACCCGGAGACGGG  >  NC_000913/525094‑525560                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  cnGGACGGCAACCGGAGCGAGACACAGTACGATGCGTGGGGAAAGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGCCCGGAAAACTCACACAGAATGAGGATg                                                                                                                                                                                                                           >  1:170138/1‑250 (MQ=255)           aCCGGAGCGAGACACAGTACGATGCGTGGGGAACGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGCCGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTc                                                                                                                                                                                                                >  1:88330/1‑251 (MQ=255)                           gTACGATGCGTGGGGAAAGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCATCCTCTGGTACTANg                                                                                                                                                                                                <  1:169972/251‑1 (MQ=255)                                  gCGTGGGGAAAGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCAGGACCGGCTGGAACAGCAGGGCGGCTTTGACGGGCGGACGCAACGGTATCATTATGACCTGACCGGAAAAATCACACAGAATGAAGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATc                                                                                                                                                                                         >  1:190496/1‑251 (MQ=255)                                                     gCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCCTTATGACCTGACCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCCTCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGc                                                                                                                                                                      >  1:90073/1‑251 (MQ=255)                                                          acGCAGGGCGGGCTGACGCGCAGTCTGGAGTACGATGCTGACGGACGTGTCATCTGCCTGTCCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCCGGGGGGACTTGACGGGCGGACGCAACGTTAGCATTATGAACTGACCGGAAAACTCAAACAGAATGAGGATGAGGGCCTTGTCCTCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACggg                                                                                                                                                                 >  1:209274/1‑250 (MQ=255)                                                                                                            catcaGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGCCGGGCGGACGCAACGTTATCATTATGACCTGCCCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGgctggctg                                                                                                                >  1:64774/1‑250 (MQ=255)                                                                                                                                aCGGCAGCCACAGCGTCTTCAGTTACGATGCCCTGGACCGGCTGGTACACCAGGGCGGCATTGACCGGCGGACCCAACGTTATCATTATGACCCGACCGGAAAACTCACCAAGAATGAGGATGAGGGACTTGTCATCCTCTGGAACTACGATGAATCGGACCGTATCACTCACCCCACGGTGACCGGAGAAACCGCAGAGCAGTGGCAGTATGATAGCCACGGCTTGCTTACAGACATCAGACACCTGAGc                                                                                           >  1:195346/1‑251 (MQ=255)                                                                                                                                  ggCAGCCACAGCGTCTGCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCGGACCGGAAAACTCACACAGAATGAGGAGGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACggctg                                                                                                                    <  1:199288/224‑1 (MQ=255)                                                                                                                                        cacaGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACCGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGcc                                                                                    >  1:199301/1‑250 (MQ=255)                                                                                                                                                                       ggCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGAt                                                    >  1:172415/1‑251 (MQ=255)                                                                                                                                                                                                                         gacctgaccGGAAAACTCACACAGAATGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACAATAAAGGCCGCCTGGCCGGCGAATGCCAGACGGTGGAGAACCCGGAGACggg  <  1:213577/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  CCGGACGGCAACCGGAGCGAGACACAGTACGATGCGTGGGGAAAGGCGGTCAGCACCACGCAGGGCGGGCTGACGCGCAGTATGGAGTACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAGTGAGGATGAGGGACTTGTCATCCTCTGGTACTACGATGAATCGGACCGTATCACTCACCGCACGGTGAACGGCGAACCGGCAGAGCAGTGGCAGTATGATGGCCACGGCTGGCTGACAGACATCAGCCACCTGAGCGAAGGCCACCGTGTTGCCGTCCACTATGGCTATGACGATAAAGGCCGCCTGACCGGCGAATGCCAGACGGTGGAGAACCCGGAGACGGG  >  NC_000913/525094‑525560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑