Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,767	G→A	C270C (TGC→TGT)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,767	0	G	A	100.0%	17.8 / NA	8	C270C (TGC→TGT)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

GCACGGTTGAGATGGCGATACCACGGGATATTCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGCCTTCTTTCAGCGCCTTAACGATCCCCTGATAGCGCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAG  >  NC_000913/3372525‑3372990                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                gCACGGTTGAGATGGCGATACCACGGGATATTCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGACAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcgacacagcgg                                                                                                                                                                                                                         <  1:194499/251‑1 (MQ=255)      gTTGAGATGGCGATACCACGGGATATTCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCGGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGcgacacagcggcacag                                                                                                                                                                                                                    <  1:110481/251‑1 (MQ=255)                                         tagtaCTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAGCTCACCCGCGCTCTTGCATCAACTTCTGGGCCAGCGCCTTCAGTTCTGTCAGATATTTTTCATCTACCGGTCCAAACGGTTTGcgacacagcggcacag                                                                                                                                                                                                                    <  1:195928/216‑1 (MQ=255)                                                                            cccACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCAGGTAGTGGCGCGCGAGGGGAAACAACTCACCCGCGCTCTCGCATCAACTGCTGAGACAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGACACAGCGGCACAGAAACGACATCCCTATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAAt                                                                                                                                              >  1:32601/1‑251 (MQ=255)                                                                                                                           ccccTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCAGCAACTGCTGGGCCAGCGCCTTCAGTTCTTGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGACACATCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCTTTTTTATCATTAAATCGATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGc                                                                                               <  1:200786/251‑1 (MQ=255)                                                                                                                                  cccGGTAGGGGCGAGCGAGGGGAAACNACTCACNNGCNCTATTGCATCAACTGCTGGNCCNNNGNNNTNCGTTCNGGNNGATCTTTTTNATCTACCGGTCCAAACGGTTTGCGACACAGCGGCACAGAAACGACATCCATATNATGGAGGACNNTTTTCAGGCCGCGGAATACGCNCGTNNTNNTNCGTnaan                                                                                                                                                  >  2:45907/1‑192 (MQ=255)                                                                                                                                                                 ccccGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTGCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGACACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGCCTTCTTTCAGCGCCTTAACGATCCCCTGAGAGCGCCAg                                                         <  1:45907/250‑1 (MQ=255)                                                                                                                                                                        ctctTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGACACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCan                                                                                                                      <  1:43374/183‑2 (MQ=255)                                                                                                                                                                                  aaCTGCTGGGCCAGCGCCGTCAGTTCTGGCAGATATTTGTCATCTACCGGTCCAAACGGTTTGCGACACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTGGATCAGTAAATCAATGACTGTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGCCTTCTGTCAGCGCCTTAACGATCCCCTGATAGCGCCAGCCCATGATGTTGTAGGt                                        <  1:105430/251‑1 (MQ=255)                                                                                                                                                                                                                          cATCTACCGGTCCAAACGGTTTGCGACACAGCGGCCCAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGAATTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGCCTTCTTTCAGCGCCTTAACGATCCACTGATAGCGCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCag  >  1:50633/1‑249 (MQ=255)                                                                                                                                                                                                                                            gTTTGCGACACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAg                                                                                                                      <  1:98634/115‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                GCACGGTTGAGATGGCGATACCACGGGATATTCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGATATCGCCTTCTTTCAGCGCCTTAACGATCCCCTGATAGCGCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAG  >  NC_000913/3372525‑3372990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑