Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,360,172 G→A M325I (ATG→ATA)  gltD → glutamate synthase subunit GltD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,1720GA88.9% 14.3 / ‑6.2 9M325I (ATG→ATA) gltDglutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (4/4);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTACCGAAATGGGCGAACCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCG  >  NC_000913/3359950‑3360370
                                                                                                                                                                                                                               |                                                                                                                                                                                                      
cGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGTGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TACCGGGTTcccn                                                                                                                                                                                             <  1:41309/234‑2 (MQ=255)
               cGTGGACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAACCGCGCCGAACCGTTCGTAAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TACCGGGTTCCCGCCGCGAAGTGAAAAACGAGCGGGAAGAAGGCg                                                                                                                                                             >  1:147184/1‑251 (MQ=255)
                     cGCAGCGCTGCCGTTCATCATCGCCAATACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGCTCGTCAGCATGGAAGGCAAACGAGTGGTGGTCATTGGCGGGGGCGACAATGCGATGGACTGCGTGCGTAAGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGGGCCTATCGTCGTGATGAAGAGAACA‑AAAAGGGTTCCCGCAGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTagagg                                                                                                                                                       >  1:30768/1‑250 (MQ=255)
                     cGCAGCGCTGCAGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGTTGACACCGGCGACGACACGTTCGTCAGCGTGGAAGGCAAAGGCGTGGTGGTCGTTGGGGGTGGCGACACTGGGATGGACTGCGGGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TACCGGGTTCCCGCCGCGGAGTGAAAAACGCGCGGGAAGAAGGCGGAGAGt                                                                                                                                                       >  1:30770/1‑251 (MQ=255)
                                   tCCTCATCGCCAACACAAAACGGTTAATGGGGTTTGGTGAAACCCACGACGAACCGTGCGTCATCATGGAAGGGAAACGCGTGGGGGGCCTTGGCGGTGACTACACTGCGATGGACTTCGCGCGTACTTCCGGGCGCGAGGGAGCGAAGCACGTTACCTGGGCTTACTGGCGTGATGAA‑AAAAGACTACCGGGTTCCCGCCGCGAAGTGAAAAAcgcgcgn                                                                                                                                                                       <  1:25213/221‑2 (MQ=255)
                                                                                                                                   gTGGCGACCCTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TACCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATGGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTACCGAAATGGGCGAACCGGACGCCAAAGGCCGTcgc                                         <  1:57499/251‑1 (MQ=255)
                                                                                                                                                 aTGGACTGCGTGTGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTCCCTGTGCCTATCGGCGGGATGAAGAGAACA‑TGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTACCGAAATGGGCGAACCGGACGCCAAAGGcc                                              <  1:30042/232‑1 (MQ=255)
                                                                                                                                                                    ccgggcgcCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TACCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTACCGAAATGGGCGAACCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATc        <  1:13509/247‑1 (MQ=255)
                                                                                                                                                                          gcCAGGGAGCGAAGCACGATACCTGTGCCTATAGTCGTGATGAAGAGAACA‑AACCAGGTGCCCGCCGCGAAGTGAAAAACGCGCGGGAAGCAGGCGTCGAGATAAAATTCAACGTACAGACGTTGGGTATTGAAGTGAACGATAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTAACGAAATGGGCGAACCGGACGCCAAAGGCCGTCGCCGCGCGGAGCTAGTTGCAGGTCCCGAACATATCGTTCCg  >  1:158374/1‑251 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                      
CGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACA‑TGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGTACCGAAATGGGCGAACCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCG  >  NC_000913/3359950‑3360370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑