Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,358,749	G→A	G1342D (GGC→GAC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,358,749	0	G	A	100.0%	34.3 / NA	13	G1342D (GGC→GAC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/10);  total (3/10)

CTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTG  >  NC_000913/3358531‑3358976                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    cTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCCTGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCCCCTTCCGCAGCCATGAAGCAAGAATTATCCGCCACACCTGCCTGTATGGCGCGACCGGGGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGgcgtgcgtc                                                                                                                                                                                                        >  1:80502/1‑247 (MQ=255)   tCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAATCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGGATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTaan                                                                                                                                                                                                      <  1:21439/248‑2 (MQ=255)            ccGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTTCTCCGGGTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCCGTATGGCGCGACCGGGGGTCGGCTGTATGCCGGCGACCGCGCGGGGGGAAGTTTCGGGGTGCGTAACTCCgggggtatc                                                                                                                                                                                          >  1:94719/1‑244 (MQ=255)               cAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCAcc                                                                                                                                                                                       <  1:216386/251‑1 (MQ=255)                                  gtgGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGcn                                                                                                                                                                    <  1:41373/251‑2 (MQ=255)                                                                         tGCCAACGACTATGTCGGTAAGGGCATGGCGGGCGGCTTAATCGCCGTTCGTCCTCCGGTTGGTTCCGCCTTCCGCGGCCATTAATCAAGCATGATCGGCAACACCTGCCTGTAGGGCGCGACCGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGgcg                                                                                                                                                                                                              <  1:217219/170‑1 (MQ=255)                                                                                 aCTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGgcg                                                                                                                                                                                                              <  1:181878/162‑1 (MQ=255)                                                                                                                        ttCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGTTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATg                                                                                       <  1:220028/242‑1 (MQ=255)                                                                                                                                                                                                    cGGTGGTCGTCTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGttn  <  1:62452/251‑2 (MQ=255)                                                                                                                                                                                                     ggtggtCGTATGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCTGCGGATTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTg  >  1:49714/1‑250 (MQ=255)                                                                                                                                                                                                         gtcgtcTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGTGCATGACCGGCGGTTTCGCTTACGTtc                                                                 <  1:68257/183‑1 (MQ=255)                                                                                                                                                                                                             tcTGTATGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTtgtg                                                                                                                                                        <  1:178865/92‑1 (MQ=255)                                                                                                                                                                                                                   tGCCGCAGACCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCGATCACCGTGGTAGAAGTCATTGGCGACAACGTTTGTGAATATATGACGGGTGGTATCGTGTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCgn                                                                                            <  1:20818/146‑2 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    CTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATATATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTG  >  NC_000913/3358531‑3358976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑