Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,334,592	G→A	A295T (GCC→ACC)	ispB →	all‑trans‑octaprenyl‑diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,334,592	0	G	A	100.0%	23.0 / NA	9	A295T (GCC→ACC)	ispB	all‑trans‑octaprenyl‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

GAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAC  >  NC_000913/3334402‑3334818                                                                                                                                                                                               |                                                                                                                                                                                                                                   taaCGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTcg                                                                                                                                                                        >  1:29639/2‑251 (MQ=255)                                                                 caGATGATCCGTACCGCCATCGAACAGGGTACCGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAAcg                                                                                                                                                     >  1:171276/1‑206 (MQ=255)                                                                                                  ggTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCGCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCTCAGCGCTGTTCAACGCGAGCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGc                                                                       <  1:187331/251‑1 (MQ=255)                                                                                                                ggAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGa                                                         <  1:14958/251‑1 (MQ=255)                                                                                                                            ggAAGCAATGAACGCTTGTGGATCGCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                <  1:92731/248‑1 (MQ=255)                                                                                                                                                gATCTCTGGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGTCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCCCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAaca                                                              <  1:186123/214‑1 (MQ=255)                                                                                                                                                     ctTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaa                                                                                     >  1:171398/1‑186 (MQ=255)                                                                                                                                                                       cGTGCCGAGGAAGAAGCAGACAAAACCATCGCAGCGTTACCGGTGCTCCCGGACACACATTGGCGAGAAGACCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATACCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAc  >  1:30033/1‑251 (MQ=255)                                                                                                                                                                                 aagaagCCGACAAAACCATCGCAGCGTTACAGGTGCTCACGGACACCCCTTGGCGAGAAGCACTCATCGGCCTAGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTCAGATCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAata                      >  1:173228/1‑221 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                                   GAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAC  >  NC_000913/3334402‑3334818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑