Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,330,556	G→A	intergenic (+160/+26)	dacB → / ← obgE	peptidoglycan DD‑endopeptidase DacB/GTPase ObgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,556	0	G	A	100.0%	30.5 / NA	13	intergenic (+160/+26)	dacB/obgE	peptidoglycan DD‑endopeptidase DacB/GTPase ObgE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

CACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCACGACCGGGTTTTCAATGATAAAGGTCATCACATCCCAGCAGAGATCTTTCACGCC  >  NC_000913/3330414‑3330797                                                                                                                                               |                                                                                                                                                                                                                                                  cacaTNNCCNNNGCNNCAGATTATTGACAAAGTGCGCTTNGTTTATGCCGGATGCGGCGTAAACGCCTTNTNCNGCCTNNAAANTCGTGCAAATNCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGANTNATTTgg                                                                                                                                                                                                                                          >  2:159109/1‑152 (MQ=21) cacaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTgg                                                                                                                                                                                                                                          <  1:159109/152‑1 (MQ=255)      ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAAtctn                                                                                                                                   <  1:173872/250‑2 (MQ=255)                                                                          ggCCTACAAAAACGTGCAAATTCAACATATTGCAATTCTCGTGTTGGCGTGAGAAGCGTAGCGGATCAGACTGATGTGGCGTTTATCTTCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTc                                                                                                                                                                             <  1:35357/140‑1 (MQ=255)                                                                                cAAAATCGTTCAAATTCAACCTATTGCAATTCTCTTGTAGGCCTGATAAGCTTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTGCTTAACGCTTGTAAATGAACTCATCGCCTTCTTCGTCGTCTTCGTCCCAGCTGTCATCCCAGTCGTCATCATCCGCTTCAGCAGTCTCTTCAAGCTTCTTGCGATGATAATCATCCCACATGAATTCGTCTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCAc                                                        <  1:29955/251‑1 (MQ=255)                                                                                cAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCCGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGCACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATACCAGTCTTCATCATCCTCTTCAGCAATCTTTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTg                                                                                 >  1:125893/1‑226 (MQ=255)                                                                                cAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTTATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCATTCTGCAGCATGCTCTGCATCAATCTCGTCAAGCTGCTGGCGATGATAGTCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTGCTTCAGCCTGCAc                                                        <  1:195296/251‑1 (MQ=255)                                                                                cAAAAGCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAAGCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCAc                                                        <  1:29950/251‑1 (MQ=255)                                                                                          cAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGAGTAGCGCATCAGACTGATTTGGc                                                                                                                                                                                                                                         >  1:126525/1‑64 (MQ=255)                                                                                            aaTTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCACGACCGGGTTTTc                                            <  1:46196/251‑1 (MQ=255)                                                                                                      atTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTGCGTCAGCCTGCACGACCGGGTTTTCAATGATAAAg                                  <  1:187527/251‑1 (MQ=255)                                                                                                                        gCCTGATAAGCGTAGCGCATCAGACTGATTTGGCGTTTCTCATCAGTGCTTAACGCTTGTAAATGAACTCAACGCCTTCTTAGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTAAGCAATCTCTTCACGCTGCTGGCGATGATAATCATCCCACATGCATTCGCCTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCCCGACCGGGTTTTCAATGATAAAGGTCATCACATccccgc                  >  1:209077/1‑246 (MQ=255)                                                                                                                                      gcgcATCAGACTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCACGACCGGGTTTTCAATGATAAAGGTCATCACATCCCAGCAGAGATCTTTCACGcc  >  1:75904/1‑251 (MQ=255)                                                                                                                                               |                                                                                                                                                                                                                                                  CACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGATGATAATCATCCCACATGAATTCGACTTTCTCTGGCTGTTTCGCTTCTTCAGCCTGCACGACCGGGTTTTCAATGATAAAGGTCATCACATCCCAGCAGAGATCTTTCACGCC  >  NC_000913/3330414‑3330797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑