Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,323,623	G→A	R150C (CGT→TGT)	glmM ←	phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,323,623	0	G	A	100.0%	23.5 / NA	10	R150C (CGT→TGT)	glmM	phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/2);  total (8/2)

CTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGC  >  NC_000913/3323393‑3323866                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTg                                                                                                                                                                                                                                 >  1:3544/1‑251 (MQ=255)    gAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGCTTACACCGTTTGGCTCACCCCCGATAGAGATAACGTTCGCCCCCCGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACACTCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTg                                                                                                                                                                                                                                 >  1:116305/1‑248 (MQ=255)           cgAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATCACGTTCGCACCCAGTTCGCGCAGCCCGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGATTTGCAAACCTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAAc                                                                                                                                                                                                                       >  1:156431/1‑251 (MQ=255)                                                cacCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGCTGTGATAAGTCGCACCGTTTGAACAATCCACCACAATCTTCAGCTCACTGAGGCTCAGTTCGATCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGcc                                                                                                                                                                                  >  1:123038/1‑251 (MQ=255)                                                                             aCGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGcc                                                                                                                                                     <  1:9213/251‑1 (MQ=255)                                                                                                                                                                                             cTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGACCGGTGtt                                     >  1:82726/1‑251 (MQ=255)                                                                                                                                                                                                  cAAAACTCGATATAGAGACCCGCGGCATCAACGATACAGCAGGCTTTAACCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGCTATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGGCCGGTGTTGGCAt                                >  1:180363/1‑251 (MQ=255)                                                                                                                                                                                                        tCGATATAGCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTATGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTAGATGGCCTCTTCTACCGCATCCGGCCGTTTGGTGCCGTCGATAGAGACGAATTTAACGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGGCAGGTGTTGGCATCGGGcc                          >  1:218044/1‑251 (MQ=255)                                                                                                                                                                                                                gCGACCCGCGGCATCAACGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTACTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACaa                                                                                       >  1:98904/1‑182 (MQ=255)                                                                                                                                                                                                                                aCGATACAGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGc  <  1:8593/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACGCAGCTGATCTCCTTTTCCATTTCCGCTTCGATGGCCTCTTCTACCGCATCCGGCAGTTTGGTGCCGTCGATAGAGAAGAATTTAATGCCATTATCGTAGAACGGGTTATGCGATGCAGATATCACAATTCCGGCCTCTGCGCGGAAGGTACGCGTCAGATAAGCCACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGC  >  NC_000913/3323393‑3323866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑