Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,302,451	C→T	L323F (CTT→TTT)	ubiU →	ubiquinone biosynthesis protein UbiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,302,451	0	C	T	90.0%	19.0 / NA	10	L323F (CTT→TTT)	ubiU	ubiquinone biosynthesis protein UbiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (3/6);  minor base G (0/1);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTATCACGCGCTGGAAGAACCAACCAGTCTCAATACCCTGGAACTGCTGCCGGAGTTAATGGCGGCGAATATTGCTTCGGTGAAAATTGAAGGCCGCCAGCGTAGCCCGGCGTATGTCAGCCAGGTGGCGAAAGTCTGGCGTCAGGCTATCGACCGTTGTAAGGCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCG  >  NC_000913/3302210‑3302633                                                                                                                                                                                                                                                  |                                                                                                                                                                                       cTATCACGCGCTGGAAGAACCAACCAGTCTCAATACCCTGGAACTGCTGCCGGAGTTAATGGCGGCGAATATTGCTTAGGTGAAAATTGAAGGCCGCCAGCGTAGCCCGGCGTATGTCAGCCAGGTGGCGAAAGTCTGGCGTCAGGCTATCGACAGTTGTAAGGCCGATCCGCAAAACTTTGTACAGCCAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCat                                                                                                                                                                               >  1:14527/1‑251 (MQ=255)                                                                                         gAAGCCCGCCAGCGTAGCCCGGCGTATGTCAGCCTGGAGGCGAAAGGCTGGCGTCAGGCTATCGACCGTTGTATGGCGGATCCGCAAAACTTCGGACCGCAGAGCTCGTGGATGGAGACGCGCGGGGCGATGTCCGAAGGCATGCAAACCACGTGTGTCGCATAGCACCTTAAATGGCAGTGAGAAAATCAATGAAATATGGCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAAttt                                                                                       <  1:98750/251‑1 (MQ=255)                                                                                                              gCGTATGTCAGCCAGGTGGCGAAAGGCTGGCGTCAGTCTATCGACCGTTGTAAGTCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTGTGGTCGCATATCTCCGTAAATGGCAGTGAGAAAAGCAATGAAAGATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACcagc                                                                  <  1:206631/251‑1 (MQ=255)                                                                                                                      cAGCCAGGTGGCGGAAGGCTGGCGTCAGGCTATGGACCGTTGTAAGGCCGATCCGCAAAACTTTGTACGGCAAAGGTCGTGGATGGAGACTCTCGGGTCGATTTAAGAAGGCACGCAAACCTCTTTTGGCGCATATGACCGTAAATGGCAGTGAGAAAAGCa                                                                                                                                                   <  1:218320/162‑1 (MQ=255)                                                                                                                                                     aTCGACCGTTGTAAGGCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATcagc                                                                                <  1:164023/198‑1 (MQ=255)                                                                                                                                                               gTAAGGCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGCCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCATCTCACCGTAAATGGCAGTGAGAAAAGCAATGAAATCTTCCTTAGCGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCAACAGCAGCGCCGACGAGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCggg                 >  1:121475/1‑251 (MQ=255)                                                                                                                                                                   ggCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAACCCACTTTTGGCGCATATCACCGTAAATGGCAGTGAGACAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTCTCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc             >  1:83495/1‑251 (MQ=255)                                                                                                                                                                              aaaaCTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGTCCAAAAGAGACGCTGGAAGAATTGTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGAGTTATCTTGGGGAAGCTGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCg  <  1:109546/251‑1 (MQ=255)                                                                                                                                                                                        aCCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCGTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCg                   <  1:150856/224‑1 (MQ=255)                                                                                                                                                                                                           gagaCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTTTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAcc            <  1:17030/212‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                       CTATCACGCGCTGGAAGAACCAACCAGTCTCAATACCCTGGAACTGCTGCCGGAGTTAATGGCGGCGAATATTGCTTCGGTGAAAATTGAAGGCCGCCAGCGTAGCCCGGCGTATGTCAGCCAGGTGGCGAAAGTCTGGCGTCAGGCTATCGACCGTTGTAAGGCCGATCCGCAAAACTTTGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCG  >  NC_000913/3302210‑3302633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑