Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	496,491	C→T	L458L (CTG→TTG)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	496,491	0	C	T	100.0%	26.0 / NA	12	L458L (CTG→TTG)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/4);  total (8/4)

CTGGTACTGAAAGAAGGTCCGGCGGAAGATTTCGCTAACCAGGAAGCGATCGCCAAACTGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTCTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTC  >  NC_000913/496257‑496697                                                                                                                                                                                                                                            |                                                                                                                                                                                                               cTGGTACTGAAAGAAGGTCCGGCGGAAGATTTCGCTACCCAGGAAGCGATCGCAAAACTGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCg                                                                                                                                                                                                >  1:79339/1‑251 (MQ=255)                    cggcggAAGATTTCGCTAACCAGGAAGCGAACGCCAAAATGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCATATCTCTGGAAGACTACGTTTCCCGCATGCAAGAAGGGCAAGAGCCACTCTACTACCTCAC‑CGCTGACAGCTATGCGGCAGCGAAGAGCAGCCCGAACCTGGAACTGCTGCGTAAGAACGGCATCTAAGTTTTGCAGCTTTCCGACCGCATCGATGAGTGGatgatg                                                                                                                                                                             >  1:212528/1‑251 (MQ=255)                                   cTAACCAGGAAGCGATCGCCAAACTGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCGTCTCTCTGGCAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATctgactg                                                                                                                                                                >  1:104928/1‑249 (MQ=255)                                           gAAGCGATCGCCAAACTGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACgg                                                                                                                                                      <  1:185046/251‑1 (MQ=255)                                                                       cTTCTACCCATACCGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCggn                                                                                                                                    <  1:43214/241‑2 (MQ=255)                                                                                   ccGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCGGCGTAATAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCAGCGATGAGTGGATTATGATCTTGCTGACTTAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCt                                                                                                               <  1:111284/250‑1 (MQ=255)                                                                                          ttctGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCCTGAAAGAAGGGCAGGCGAACATCCAATACCT‑ACACGAAGACCGCTATGCGGCAGCGAAGAGCAGACCGACCCTCGAACTGCTGCCAAAGAACGGCATCGGAGTTTTGCTGCTTTCCGACCGCGTCGATGAGCGGATGATGAACTACCTGACAGAGCACGACGGTAAACCGTTCCAGTCGGCGTCTAAAGTTGACGAGCCGCTTGAAAAAc                                                                                                       >  1:206896/1‑251 (MQ=255)                                                                                                ncAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCAAAAGTTGACGAGTCGCTTGAAAAACTGGCTg                                                                                                 >  1:132705/2‑251 (MQ=255)                                                                                                               ggAAGACTGCGTTTCTCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgatg                                                                                     <  1:14751/248‑1 (MQ=255)                                                                                                                                            ggCAGGAGAAAATCTACTACATCAC‑AGCAGACAGCTATACGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTACAGTTGACGAGTCGCTTGAACAACTGGCTGACGCAGTTGATGAGAGAGCGAAAGAAGCGGAGAAAGCACTGACt                                                     >  1:90563/1‑251 (MQ=255)                                                                                                                                                 gagaAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGtt                                                >  1:14594/1‑251 (MQ=255)                                                                                                                                                                                                ncagcCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTTTGCTGCTTTCCGACCGCATCGATGCGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGAGGAGAAAGCACTGACTCCGTGCATCGACCGTGTGAAAGCCCAGCTCGGCGAGCGCGTGAAAGATGTc  >  1:38223/2‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                               CTGGTACTGAAAGAAGGTCCGGCGGAAGATTTCGCTAACCAGGAAGCGATCGCCAAACTGCTGCGTTTTGCTTCTACCCATACCGATTCTTCTGCGCAGACCGTATCTCTGGAAGACTACGTTTCCCGCATGAAAGAAGGGCAGGAGAAAATCTACTACATCAC‑CGCAGACAGCTATGCGGCAGCGAAGAGCAGCCCGCACCTGGAACTGCTGCGTAAGAAAGGCATCGAAGTTCTGCTGCTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAGAAAGCACTGACTCCGTTCATCGACCGTGTGAAAGCCCTGCTCGGCGAGCGCGTGAAAGATGTC  >  NC_000913/496257‑496697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑