Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,292,458	G→A	intergenic (+26/+17)	yraK → / ← rsmI	putative fimbrial adhesin YraK/16S rRNA 2'‑O‑ribose C1402 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,292,458	0	G	A	100.0%	12.5 / NA	6	intergenic (+26/+17)	yraK/rsmI	putative fimbrial adhesin YraK/16S rRNA 2'‑O‑ribose C1402 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

GGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCC  >  NC_000913/3292331‑3292622                                                                                                                                |                                                                                                                                                                     ggCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACACAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTagc                                            <  1:70761/250‑1 (MQ=255)  gCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACACAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTagc                                            >  1:92675/1‑249 (MQ=255)  gCACCGACAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACACAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTagc                                            >  1:65375/1‑249 (MQ=255)                                           ggtgAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACACAACGCATCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGGAAGacttttttg  >  1:11146/1‑245 (MQ=255)                                                                                                                 tGCGTTTTGATTAACACAACGCTTCGGCACTGTTACCCctgc                                                                                                                                            <  1:3224/42‑1 (MQ=255)                                                                                                                 tGCGTTTTGATTAACACAACGCTTCGGCACTGTTACCCctgc                                                                                                                                            >  2:3224/1‑42 (MQ=255)                                                                                                                                |                                                                                                                                                                     GGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCC  >  NC_000913/3292331‑3292622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑