Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,970	A→G	T186A (ACA→GCA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,970	0	A	G	100.0%	14.5 / NA	7	T186A (ACA→GCA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/3);  total (4/3)

CCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGA  >  NC_000913/3268795‑3269151                                                                                                                                                                                |                                                                                                                                                                                      cccATTAAAAGATATTTACAATGAAATCAGGAGGTTACACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAACTTGAATTAAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAACACGCCACAATCTCCTGGGatt                                                                                                            >  1:106088/1‑251 (MQ=255)   cNTTAAAAGATATTTACAATGAAATCCGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGTTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGattat                                                                                                          >  1:168545/1‑251 (MQ=255)                                  tttAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTACAGTTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCGGGGATTATTTACTAAAAATAACGc                                                                                          <  1:143735/235‑1 (MQ=255)                                           anTTTNTNNTNNTGGNACTGGAGATATCGTTGATCTANNCTCTCTGGANTTACGTAATGTCGATTTAGATTAT‑ATGATTTCACAGATAAACATATGGCTAATNCTATTTTAANNCCNGTTAANTNNNANNNAGNAAATTTTACTAATGCCAACNTGNNTCAGGTNAATTTTGTTAGTTCAACACAAAACGc                                                                                                                             <  2:46053/191‑1 (MQ=255)                                                                                    ntctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGttt                          >  1:128688/2‑250 (MQ=255)                                                                                                              gATTATTATGATTTCACAGATAAACATATGCCTAATACTATTTTAAATCCCTTTCAATTGAATTCAGAAAACTTTACTAATGCCAACATGTTTCCGGTTAATTTTGTTAGTTAAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCga  >  1:34418/1‑248 (MQ=255)                                                                                                                                 aTAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTGACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTgg                                                                                                                <  1:28535/119‑1 (MQ=255)                                                                                                                                                                                |                                                                                                                                                                                      CCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGA  >  NC_000913/3268795‑3269151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑