Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,210,300	G→A	P82S (CCT→TCT)	tsaD ←	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,210,300	0	G	A	100.0%	11.2 / NA	6	P82S (CCT→TCT)	tsaD	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

CGTACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACGCACATGATCGCGGGAGGCCAGTTCAGGCACGACGCCGCCGTAGTCAGCGTGCAATTTCACCTGACTATACAATTGGTTGGCTAACAAACCTTTTTCATCGTCGTAAATGGCGATGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTAC  >  NC_000913/3210090‑3210537                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              cGTACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGGAGCGCGCCGACTAATCCAGAGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGt                                                                                                                                                                                                        <  1:90809/250‑1 (MQ=255) cGTACTGACCAATGCCAGTCACGCTGATGAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGTGTTATCTTCCAGCAGCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGACCGTCCCAGGCAAACGCCAGAGAACTCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGAGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGtt                                                                                                                                                                                                       <  1:197990/251‑1 (MQ=255)          cAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAACCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGCACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGAGcc                                                                                                                                                                                                                                            >  1:119847/1‑205 (MQ=255)                                                                                                                               gtACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGAGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAAcn                                                                                                                                                                                                  <  1:174465/130‑2 (MQ=255)                                                                                                                                                                                    ncgcCAACCAGTAGCGCGCCGACTAATCCAGAGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCAGCCTGGATCAACGGTACGGTTTTACGCACATGATCGCGGGAGGCCAGTTCAGGCACGACGCCGCCGTAGTCAGCGTGCAATTTCACCTGACTATACAATTGGTTGGCTAACAAACCTTTTTCATCGTCGTAAATGGCGATGCCGGTTTCATCGCAgg                     >  1:24279/2‑250 (MQ=255)                                                                                                                                                                                                      ccGACTAATCCAGAGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACGCACATGATCGCGGGAGGCCAGTTCAGGCACGACGCCGCCGTAGTCAGCGTGCAATTTCACCTGACTATACAATTGGTTGGCTAACAAACCTTTTTCATCGTCGTAAATGGCGATGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTac  <  1:80962/251‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              CGTACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACGCACATGATCGCGGGAGGCCAGTTCAGGCACGACGCCGCCGTAGTCAGCGTGCAATTTCACCTGACTATACAATTGGTTGGCTAACAAACCTTTTTCATCGTCGTAAATGGCGATGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTAC  >  NC_000913/3210090‑3210537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑