Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,209,831	G→A	A238V (GCC→GTC)	tsaD ←	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,209,831	0	G	A	100.0%	18.3 / NA	8	A238V (GCC→GTC)	tsaD	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/2);  total (6/2)

ACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGACGCGCGTAGAACACTTCGCCGCGGCGTTTTTTCATCATTTCAGCCAGCTTCGCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAACGGCCCGCCAGGATAATCCAGCCCCAGCAGCTTCGCGGTTTTATCAAACGCTTCCCCGGCGGCATCATCG  >  NC_000913/3209607‑3210070                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                nccGCACCATTCCGGCACAGGCGATCATCGCGCCGTTATCCGTACAAAATTCCGGACGCGCGTAGAACACTTCGCCGCGGCGTTTTTTCATCATTTCAGCCAGCTTCGCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTgg                                                                                                                                                                                                                       >  1:18804/2‑251 (MQ=255)                                                         cgcgcgTAGAACACTTCGCCGCGGCGTTTTTTCATCATTTCAGCCAGCTTCGCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCAGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGTGTATCGCCCACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGgaga                                                                                                                                                                >  1:152746/1‑250 (MQ=255)                                                                                     ttttCATCATTTCAGCCAGCTGCGCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCTGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGc                                                                                                                                                                      <  1:90500/216‑1 (MQ=255)                                                                                                            gCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAgcg                                                                                                            >  1:120793/1‑251 (MQ=255)                                                                                                                 aaacgtccggtaacccctccccccgcccgcCAGGACCAGTCGCTTAAAGCCCGTCTGAGCCAGCGCCCGCTTGCCCTTAATCAGCAGCGTATCGACCACCGCTTCTACAAAGACGCGGGCGATATGAGCACGCGTCTTGTCGGTGGTGCCGTTGTCACGAATGGTTTTTGCCGCGAAGGTTTGCAGGCCGAAGAAGCTGAAACCCAGCCCCGGCCGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCgg                                                                                                       <  1:140612/229‑1 (MQ=255)                                                                                                                        nggTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACaaa                                                                                                               >  1:62979/2‑236 (MQ=255)                                                                                                                                                                                  cccGCTTGCACTTAATCATCAGCGTATCGACAACCGCATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGCCGTCGGTGCCGTTGTCACGACTGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggtcgg                                                                                                                                     >  1:89796/1‑156 (MQ=255)                                                                                                                                                                                                                      cATCTTCAAAGACGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGAAACGGCCCGCCAGGATAATCCAGCCCCAGCAGCTTCGCGGTTTTATCAAACGCTTCCCCGGCGGCATCATCg  >  1:202840/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGACGCGCGTAGAACACTTCGCCGCGGCGTTTTTTCATCATTTCAGCCAGCTTCGCCCGTAACGTACGGTTAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAACGGCCCGCCAGGATAATCCAGCCCCAGCAGCTTCGCGGTTTTATCAAACGCTTCCCCGGCGGCATCATCG  >  NC_000913/3209607‑3210070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑