Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,637	G→A	S205N (AGC→AAC)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,637	0	G	A	100.0%	32.7 / NA	16	S205N (AGC→AAC)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/5);  total (11/5)

CACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGT  >  NC_000913/3208431‑3208878                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  cACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCTAGCTGATCCTCGCACCTGTATCACCGTCCAACTCTGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCGATCAATCACAACCTGACGACAGCTGTTCGCGCATCATTGGCTCTTACATCATGTGGACGGCGATTTTTGCCGACTGCGCTACCAACGCCATTTTCTTGACGGCGAGGTCACCAAACTTGCTGTTAATTg                                                                                                                                                                                                       <  1:188871/251‑1 (MQ=255)                          aTGTTCTCCGAGCTGATCCTCGCCCCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTGCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGGCGGCGATGGCACCAAACTTGCTGATAATTGGACTGATGAAAAGCGCATCTcacgc                                                                                                                                                                              <  1:121615/251‑1 (MQ=255)                                                          acCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCTACCGCTCTATCAATCACAACCAAACGCCCGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGCTGGGGATTGCTGCCGACTTCGTGACCTACGCCCTTTTCTTGACGGCGATGGCCCCAAACTTGCTGTTTATTGGACTGATGACAAGCGCATCTCACGCCACGCTGGGGTGGGGCGACTGGTTCCTCggg                                                                                                                                               >  1:212737/1‑250 (MQ=255)                                                                              gtgCGGGGATTATCTCTCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGCCTGGGTCCTCGGGATGTTGCCGCTCAGCAtttt                                                                                                                           >  1:73814/1‑250 (MQ=255)                                                                              gtgCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATAACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCCTGTGGATGGGGATTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGCCGGCGATGGCACCACACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTg                                                                                                                                                                  >  1:80059/1‑211 (MQ=255)                                                                                                    catcCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTTTTAATTGGAc                                                                                                                                                                                                    <  1:58446/155‑1 (MQ=255)                                                                                                       ccGTAACCTGCCACCGCTCTATCAATCACAACNAAACGACAGCNGTTNNCGCANCNTNGGCTCGTACATCATGTGGATGGGGATTGTNGCCGACTGCGTGACCAACGCCATNNTCNNNACNNCGATg                                                                                                                                                                                                                             <  2:163068/127‑1 (MQ=255)                                                                                                       ccGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCCTTGGCTCCTACATCATGTGGATCGGGCTTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATg                                                                                                                                                                                                                             >  1:163068/1‑127 (MQ=255)                                                                                                        cGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGACCATCCTGAGGAAGGCGATACTTGCCGCCTGCGTGACCAACCCCATTTTCTTGACGGCGCTGGCACCAAACTTGCTGCTCATTGGACTGATGAAAAGCGCATCTCACGCCACACTGAGTTGGGGCGACTGGATCCTCGGGATGTTGCCGCTCAGCATTTTACTGGATCCGCTGGTTCCCtggctggc                                                                                                >  1:140927/1‑251 (MQ=255)                                                                                                                                 cacaACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTCCCTCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTcccgccacg                                                                                                                                                                          >  1:144407/1‑152 (MQ=255)                                                                                                                                                                       tACATCATGTGGCTGGGGATTGTTGACGTCTGGGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGACACGCTGCGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctgg                                                                                                     >  1:65933/1‑183 (MQ=255)                                                                                                                                                                                            gttgCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACACTGCGTTTGGGCGACTGGTTCCTCGGGGTGTTGCCGCTCAGCATTTTACTGGTTCTGCTGATTACCAGGCTGGCTGACGAGATGTACCCGCCGGTACTGAAGTCTGGCAATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAgagataggaagatc             >  1:844/1‑237 (MQ=255)                                                                                                                                                                                            gttgCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCAAGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGtt                                                                                                           >  1:147999/1‑156 (MQ=255)                                                                                                                                                                                            gttgCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACNGATNAAAANCGNATCTCACGCCACGCTNAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTNNTNNNTNTGCTGGtt                                                                                                           <  2:147999/156‑1 (MQ=255)                                                                                                                                                                                               gCCGACTGCGTGACCAACGCCATTTTCTTGACGGCGCTGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATc                                                          >  1:185236/1‑202 (MQ=255)                                                                                                                                                                                                      gCGTGACCAACGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGCTGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCTTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGt  >  1:221234/1‑251 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  CACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGT  >  NC_000913/3208431‑3208878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑