Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,841	C→T	G376G (GGC→GGT)	nupG →	nucleoside:H(+) symporter NupG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,841	0	C	T	100.0%	20.8 / NA	10	G376G (GGC→GGT)	nupG	nucleoside:H(+) symporter NupG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

CGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAAC  >  NC_000913/3106654‑3107079                                                                                                                                                                                            |                                                                                                                                                                                                                                               cGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCGGATGATGACTAACGGCTTCGGCTGGATCCCCGGCGGCATCGTGAGCGGTAAAGTTGGTGAGATGGACACCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTcgc                                                                                                                                                                                  <  1:64574/250‑1 (MQ=255)  gatgatCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTAGTCGAAAAAGAAGTCAGCACGGCAATTAGCGCCAGTGCAAAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGt                                                                                                                                                                                >  1:103053/1‑251 (MQ=255)        cGTTTACGGTTGCGCATTCGACGTCTTCAACAGCGCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAAGTCGCGCCAGTGCACAAGGTATGTTCCTGATGATGACTAACGGCTTCGGCTTGATCCTCGGCCGCCTCGTGAGCGGTAAAGTTGCTGAGATTTACACCCAAAACGGTATTACCGACTGGTAGATCGGATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATgg                                                                                                                                                                          <  1:204714/251‑1 (MQ=255)                                                         gTCGAAAAAGACGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGt                                                                                                                         >  1:74935/1‑251 (MQ=255)                                                                                                     gATGTTCCTGATGATTACTAACGGCTTCGGCTGTATCCGCGGCGGCAGCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGTATTACCGACTGGCAGACCTTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGGGTTCATGGCGATGGTCAAATATAAACACGGTCGTGTCCCGACAGGCACTCAGACGGTTATCCACTAATTACGCTAAGAAAAACTGGTCGCCAGAAGGTGAc                                                                             <  1:140637/251‑1 (MQ=255)                                                                                                                                                           cGGTAAAGTTGTTGAGATGTACACCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGt                                                                                                                                                                                              <  1:95597/84‑1 (MQ=255)                                                                                                                                                                        gagaTGTACACCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTc           <  1:74023/250‑1 (MQ=255)                                                                                                                                                                               ncacCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATaa    >  1:135030/2‑250 (MQ=255)                                                                                                                                                                                cacCCAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACGGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAac  <  1:14986/251‑1 (MQ=255)                                                                                                                                                                                    cAAAACGGTATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGttt            >  1:175886/1‑237 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                               CGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAAC  >  NC_000913/3106654‑3107079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑