Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,031,709	G→A	D115N (GAT→AAT)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,031,709	0	G	A	100.0%	11.3 / NA	6	D115N (GAT→AAT)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/5);  total (1/5)

GTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGCGCCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGG  >  NC_000913/3031489‑3031905                                                                                                                                                                                                                             |                                                                                                                                                                                                     gTACGCAGGTGCTGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGAGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGTGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGAATATCGGCCTGCTGGGGATATTTGGTGcc                                                                                                                                                                         <  1:214923/250‑1 (MQ=255)      cAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGAATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                                                                                                                                                                   <  1:91198/251‑1 (MQ=255)                                                                                                            tCACATTATCGCAATGTATCGGTATCGGCCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATTATATCCACTGCTATGAACCCGAATATCgg                                                                                                                                                                                               <  1:59388/121‑1 (MQ=255)                                                                                                                                           cTTTATGGGTATCCGCCGGCCGGTGATTATGTCGGTGCCCTTTGCTGCTTTACCACCAATGATATCCATTGGGATGAACCCGAATATCGGCCTGCTGGGGATATTTGGTGCCACTACCGCCGTGGTTTTGATCCCCACATTATGAGCGCTGCTTATCGTTCGCTTGATTCCTTTATTCCCGCCACTGGTTTCCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGgc                                <  1:149915/249‑1 (MQ=255)                                                                                                                                                         gCCTGCCGGTGATTATGTCGGTGAACTTTGCTGCTGTAACACCAATGATAGCCCTTGGAATGAACACGAATATCGGCCAGATGGGGCTCTTTGGTGCAACTATCGCCGCGGGTTTTATCACCACCTTATTAGCGCCCCTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTAACGGTGTGGTTATTAATTCTATCGGGCTTAGCATCATTCAGGTGGGTCTTGACTGGGCCGCCGGAGGGAAAggg                >  1:207508/1‑251 (MQ=255)                                                                                                                                                                        tGTCGGTGCCCTTTGCTGCTGTAACCCCAATGATAGCCATTGGGATGACCCCGAATATCGGCCTGCTGGGGATATTTGGTCCCACTTTCGCCGCGGTTTTTATCACCACATTATTAGCTCCCCTTCGCGGTCGTTTTATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTGAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATgg  <  1:219471/250‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                     GTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGCGCCACTTATCGGTCGCTTGATGCCTTTATTCCCGCCACTGGTTACCGGTGTGGTTATTACTTCTATCGGGCTTAGCATCATTCAGGTGGGTATTGACTGGGCCGCCGGAGGTAAAGGGAATCCGCAATATGG  >  NC_000913/3031489‑3031905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑