Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,169	G→A	L370L (CTG→CTA)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,169	0	G	A	100.0%	43.0 / NA	15	L370L (CTG→CTA)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)

ACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTG  >  NC_000913/3017005‑3017409                                                                                                                                                                     |                                                                                                                                                                                                                                                 aCTAAAACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGATGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCACCCGACctgct                                                                                                                                                                                       >  1:14463/1‑224 (MQ=255)                                gcgcACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGTCCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCa                                                                                                                                                    <  1:198364/228‑1 (MQ=255)                                  gcACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGc                                                                                                                           >  1:60051/1‑251 (MQ=255)                                            gTGAAGCGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACtt                                                                                                                 >  1:76193/1‑251 (MQ=255)                                              gAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGAcc                                                                                                                                                                                           >  1:58052/1‑175 (MQ=255)                                                                   cGTGCGCTGTTCCCGCTCTCCATCCATGTTGCAGAAGTTCTCTCTCGCGCATTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGACGGCTCCGACGCCTGGGGACTTGa                                                                                                               >  1:75873/1‑230 (MQ=255)                                                                      gcgcTGTTCCCGCTCTCCATCAATGTNGCAGCANNTCNCTCTCGCGCCTTTGACGGCNAANNNCNNNTNTCTTANTCNNGTGGTGCCANTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGNAACCGACCTNNTGAAACCTGGCGGCTATCTGCNCTTNNGNNcntgcntgn                                                                                                                                                 >  2:46523/1‑192 (MQ=255)                                                                                cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGgc                                                                              >  1:94812/1‑250 (MQ=255)                                                                                                 ncagcagTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCAt                                                            >  1:43710/2‑251 (MQ=255)                                                                                                  cagcagTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATg                                                           >  1:6278/1‑251 (MQ=255)                                                                                                  cagcagTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTAGTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCGGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATg                                                           <  1:203976/251‑1 (MQ=255)                                                                                                     cagTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCCGCTAACTATCCGCGATCTTTTTGCTACAGGTATTCGCCCTATTACTATGGCAACCGACCCGCTGAACCCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACCGACTGGCAGCAGATGCGTTAAcactgga                                                         >  1:123204/1‑244 (MQ=255)                                                                                                            ctctcGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATacac                                                   <  1:46523/249‑1 (MQ=255)                                                                                                                                                 tCCGGTGGTGACAGTCAGCTAACTATCCGCGATAGTTTGGATACAGGTATTCGCCCTATGACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACTATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTgg             <  1:190131/250‑1 (MQ=255)                                                                                                                                                           ncAGTCAGCTAACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTg  >  1:56356/2‑251 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                                                                 ACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTG  >  NC_000913/3017005‑3017409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑