Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,932	G→A	T291T (ACG→ACA)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,932	0	G	A	100.0%	16.3 / NA	7	T291T (ACG→ACA)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGC  >  NC_000913/3016804‑3017159                                                                                                                                 |                                                                                                                                                                                                                                    ntCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGAACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACACAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGa                                                                                                              >  1:20575/2‑248 (MQ=255)             gtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACACAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTc                                                                                               >  1:72567/1‑251 (MQ=255)                       aCCCGACCTTACCGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGTCCTCAAGCTGACACAAGCACTGGAAATGCTGGAACGCCTGATGTCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAACACTGACTAACACTCGCCGCACCATCAACAATAATGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTgcn                                                                                     <  1:18567/251‑2 (MQ=255)                                            cgcgTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACACAAACACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAAt                                                                >  1:144236/1‑251 (MQ=255)                                                             cTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACACAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCt                                                                                                                   >  1:187122/1‑183 (MQ=255)                                                                                                  aagaGTCATTTGATCACGTCCTCAAGCTGACACAAGCACTGGAAATGCTTGAACGCCTGATGTCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCGCGGCACCATCAACAATAAAGGCGCACGGCCTGGTGAAGAGATGTATATGTCATGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTGTGACGGCAAACTGCCCATTTCTTATTcc          <  1:137600/251‑1 (MQ=255)                                                                                                                     ccTCAAGCTGACACAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTGTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAAGAAATGCGCACTTCCTGGGGAAGAGATTTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCAGCAATGTTGCAGCATTTCTCTCTCGCTCCGTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGc  <  1:203001/240‑1 (MQ=255)                                                                                                                                 |                                                                                                                                                                                                                                    CTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTTGGCGTAAAACTGACTAACACTCTCGGCACCATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGC  >  NC_000913/3016804‑3017159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑