Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,355	G→A	G299R (GGG→AGG)	yqeA →	putative amino acid kinase YqeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,355	0	G	A	90.9%	24.2 / NA	11	G299R (GGG→AGG)	yqeA	putative amino acid kinase YqeA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (5/5);  minor base C (1/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCAT  >  NC_000913/3012153‑3012571                                                                                                                                                                                                           |                                                                                                                                                                                                                         ggTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAAAAGTGGCTAGATCGACTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGCAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGAAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGcc                                                                                                                                                                          >  1:149249/1‑251 (MQ=255)      aaaaaGTGGCCATGAACTTCGGTAAAGAGAATGAACAGTGGCTGGATCGCCTGTCATTAAGTGATGCTGAACGCTTGATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCgggtg                                                                                                                                                                     <  1:97/251‑1 (MQ=255)                              aaGAGAATGAACAGTGGCTCGATCGCCTGTCAGTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATGTAAAGGCCGGGTGGGTAATACCATCCgg                                                                                                                                                      <  1:167636/242‑1 (MQ=255)                                                                                                   ttGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCgn                                                                         <  1:61652/250‑2 (MQ=255)                                                                                                                  tgctgcCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTAGTGACGCACTGATTACCGTATTGAGCAAAGCGAAAGAACGGATTGAAGGCAAAACAGGAACGGAGATTTGTCAGTCATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGc                                                         >  1:83237/1‑251 (MQ=255)                                                                                                                                        cgccTCGTTTGCTCGCTCTCGCGCAGGGCGTGAAGCACTGATTACCGTATTGATCAAAGCGAAAGAAAGGATTTATGGCAAAACAGTAACGTTGATTGGTCAGTAAGGTAAAGGTCGGGTGGTTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCttt                                                                                                                           <  1:177083/163‑1 (MQ=255)                                                                                                                                          ccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAACGAGAAAGAAAGGCTTGAAGGCAAACCAGGAACGGTGATTTGTCAGTAATTTAACGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAAc                                                                              >  1:137542/1‑206 (MQ=255)                                                                                                                                                         ctcGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTANNTTaa                                                                                                                                                                               >  2:4092/1‑94 (MQ=255)                                                                                                                                                                    ngTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGCTTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCACATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGttt       >  1:43030/2‑251 (MQ=255)                                                                                                                                                                       gAAGCACTGATTACCGTATGGAGCAAAGCGAAAGAAAGGATTGAATGCAAAACATGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCTGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAAGAGATGGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGtc    <  1:145674/251‑1 (MQ=39)                                                                                                                                                                         ngCACTGATTACCGTATTGAGCAAAGCGAAAGAAAGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGtcat  >  1:20736/2‑251 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                         GGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCAT  >  NC_000913/3012153‑3012571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑