Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,991,428	G→A	G54D (GGT→GAT)	ygeG →	TPR repeat‑containing putative chaperone YgeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,991,428	0	G	A	100.0%	29.2 / NA	11	G54D (GGT→GAT)	ygeG	TPR repeat‑containing putative chaperone YgeG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/8);  total (3/8)

GATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGG  >  NC_000913/2991196‑2991631                                                                                                                                                                                                                                         |                                                                                                                                                                                                            naTAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGc                                                                                                                                                                                              >  1:38031/2‑248 (MQ=255)      atatAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTCGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAAttt                                                                                                                                                                                      <  1:70886/251‑1 (MQ=255)                                                    gaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCt                                                                                                                                        >  1:182673/1‑251 (MQ=255)                                                        caNCACNGNNGNNTATNATGAGCACAGAAACAATTGAANNATTCAATAANAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTNGCATTACTANNTGNTTTAANTNNNGNNNTCNTTGATAGAATATATGCATANGCNNTCGACTNCCATGAAAAGGATAATATAACGGACg                                                                                                                                                                                               <  2:46477/192‑1 (MQ=255)                                                                             gCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAg                                                                                                               <  1:6661/251‑1 (MQ=255)                                                                                                atTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATATcaac                                                                                            <  1:81814/251‑1 (MQ=255)                                                                                                                                                     gagaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCNTTCNNNNNTc                                                                                                                                                  >  2:136437/1‑144 (MQ=255)                                                                                                                                                                      ctacATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATTAATGCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGc                                                                                                                            <  1:99855/149‑1 (MQ=255)                                                                                                                                                                            ggTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTcn                <  1:136437/251‑2 (MQ=255)                                                                                                                                                                                         atatCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTGTGCATCAGTATGTCAGCCCAAAAAGAAAAATCATCAAGCATATGACCTGTACAAACTAAGTTACAATTACTTGCCGTATGATGACTATTCAGTTAGTTATCGTATGGGTCAATGTCAGATTggg   <  1:148058/251‑1 (MQ=255)                                                                                                                                                                                           atCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGATAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTgggn  <  1:51441/250‑2 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                            GATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGG  >  NC_000913/2991196‑2991631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑