Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,972,092	G→A	E166K (GAG→AAG)	tas →	putative NADP(H)‑dependent aldo‑keto reductase Tas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,972,092	0	G	A	100.0%	24.9 / NA	10	E166K (GAG→AAG)	tas	putative NADP(H)‑dependent aldo‑keto reductase Tas
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

GACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAGAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACAC‑GATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTT  >  NC_000913/2971882‑2972309                                                                                                                                                                                                                   |                                                                                                                                                                                                                           gACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTTCTTCGGCAAACGCGGTTATAGCTGGACGGAGTCGGCGCCTGCGGTTTCGCTGCTGTATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTc                                                                                                                                                                                    <  1:87373/251‑1 (MQ=255)      gggCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATGCGTTATATCGGCGTGTcgaacg                                                                                                                                                                               <  1:48226/251‑1 (MQ=255)                                                                     cAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGGCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATATGGCGGACAAAAAC‑GATCTGCcgcggactg                                                                                                               >  1:10698/1‑246 (MQ=255)                                                                                     gATTACCTCGATCTTTATCCGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGACACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAACCAC‑GATCTGCCGCGTATTGTCACCATTCAGAAccc                                                                                               >  1:78495/1‑251 (MQ=255)                                                                                                                     ncAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATCGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTAGATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGACACTGCATTTGGCGTAATGCGCTACCTCCATCTGGCGGACCAACCC‑GATCTGCCGCGTATTGTCACCATTCCGAACCCTTTCCGTCTGTTAAACCGACGTTTTGAAgg                                                                 >  1:22069/2‑248 (MQ=255)                                                                                                                           nccGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGAGGTTTCGCTGCTGGATACGCTGGGCGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAAATGCATTTg                                                                                                                                                                   >  1:26233/2‑146 (MQ=255)                                                                                                                                  aaCTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCCAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACTTCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACAC‑GATCTGCCGCGTATTGTCACCATTCAGAACCCTTCCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGa                                                     >  1:94039/1‑248 (MQ=255)                                                                                                                                           ggCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACAGCATTTGGCGTAATGCGCTACCTGCCTCTTGCGGACAAACAC‑GATCTGCCGCGTATTGTCACCCTTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTCGGTCTGGCAGAAGTCAGCCAGt                                          >  1:15946/1‑250 (MQ=255)                                                                                                                                                                                  gTTTCGCTGCTGGATACGCTGGACGAACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGCAATGCGCTACCTGCATCTGGAGGACAAA‑ACAGATCACCCGCGTATTGTCACCATTCAGAACCCTTAAAGTCTGTTCAACCGCAGTTTTGGAGTAGGTATGGAAGAAGTCAGCCAGTATGGAGGGGTCGAACTGCTGGCCTATTCGTGTCTGGGttt  >  1:35265/1‑251 (MQ=255)                                                                                                                                                                                             ggATACGCTGGACGCACTGGCAAAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACAC‑GATCTGCcgcg                                                                                                                    <  1:189255/126‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                           GACAAGGGCATCCGCCCGGATCAGGCGCTGGATCGGAAGAATATCCGCGAAGCGCTGCATGACAGCCTCAAGCGCCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCCCGACCAACTGCTTCGGCAAACTCGGTTATAGCTGGACGGATTCTGCGCCTGCGGTTTCGCTGCTGGATACGCTGGACGCACTGGCAGAGTACCAACGCGCGGGAAAAATTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACAC‑GATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTT  >  NC_000913/2971882‑2972309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑