Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,948,717 G→A I98I (ATC→ATT)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,948,7170GA100.0% 27.4 / NA 11I98I (ATC→ATT) amiCN‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

GCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTGATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCAGGTTTACATCTTCGATATCCACCACCACGCGTTCAGGGTTACTCAACGCGAACTGCTTATATTTCAGCTGACGATTTGATTCCACCGTCACGCGGGTGTAGCTGGACGCAGGCCAGACGCGCACCGCCACGACCTGGCTGACCGCAGCCAGACTGACCTGACT  >  NC_000913/2948506‑2948935
                                                                                                                                                                                                                   |                                                                                                                                                                                                                          
gCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATcccc                                                                                                                                                                                     <  1:59079/251‑1 (MQ=255)
                                        cagcagcgGGTCCTGCATATCCTGTGCATTGGCCGTATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAATGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGTCTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCAGGTTTACAGCTTCGATATccacca                                                                                                                                             <  1:29427/251‑1 (MQ=255)
                                                           tCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCGTGAGTACCGAGTTCAGGTTTACATCTGCGATATCCACCACCACGCGTTCAGGGTTAc                                                                                                                           <  1:204226/250‑1 (MQ=255)
                                                                       gCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCTGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTGCAGGTTTACATCTTCGATATCCTCCACCACGCGTTCAGGGTTACTCAACGCGAACTg                                                                                                              <  1:195397/251‑1 (MQ=255)
                                                                        ccGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGTCGCAAGGTCAAACAGCTGCGGTGTTACGGTTTGCTTTAATTCAAAAACCATCCGTACGGGTTGCGGGTCAAATTGCCCGACGCGCGCCGACGTAATGAACGTGTCGTCTGCACGAATTTGTGCCGCCATCCCCTGGAGTACCGAGTTCAGGTTTACATCTTCGATATCCACCACCACGCGTTCAGGGTTACTCAACGCGAACTGc                                                                                                             <  1:162726/251‑1 (MQ=255)
                                                                        ccGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTTCCGAGTTCAGGTTTGCATCTTCGATATCCACCACCACGCGTTCAGGGTTACTCAACGCGAACTGc                                                                                                             <  1:162724/251‑1 (MQ=255)
                                                                                    ccATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCATACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGTATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCAGGTTTACATc                                                                                                                                                           >  1:216974/1‑193 (MQ=255)
                                                                                                                                    gCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCCACTTAATGAACg                                                                                                                                                                                                                     >  1:109050/1‑87 (MQ=255)
                                                                                                                                                             ttCAANNACNNNCCNNACGGTTTGCGGGTCAAATTGCCCNACGCGCGCCGACTTAATGAACGGGTCGTCNGNANGAATNNGTGNCGCCATCCCCNTGAGTACCGAGTTCAGGTTTACATCTTc                                                                                                                                                        >  2:163918/1‑123 (MQ=255)
                                                                                                                                                             ttCAAAAACCATCCGTGCGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCTGGTTTACATCTTc                                                                                                                                                        <  1:163918/123‑1 (MQ=255)
                                                                                                                                                                                       ggTCAAATTGCCCGACGCGCGCCGACTTAATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCAGGTTTACATCTTCGATATCCACCACCACGCGTTCAGGGTTACTCAACGCGAACTGCTTATATTTCAGCTGACGATTTGATTCCACCGTCACGCGGGTGTAGCTGGACGCAGGCCAGACGCGCACCGCCACGACCTGGCTGACCGCAGCCAGACTGACCTGACt  >  1:4456/1‑247 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                          
GCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTTTGCTTTAATTCAAAAACCATCCGTACGGTTTGCGGGTCAAATTGCCCGACGCGCGCCGACTTGATGAACGGGTCGTCTGCACGAATTTGTGCCGCCATCCCCTTGAGTACCGAGTTCAGGTTTACATCTTCGATATCCACCACCACGCGTTCAGGGTTACTCAACGCGAACTGCTTATATTTCAGCTGACGATTTGATTCCACCGTCACGCGGGTGTAGCTGGACGCAGGCCAGACGCGCACCGCCACGACCTGGCTGACCGCAGCCAGACTGACCTGACT  >  NC_000913/2948506‑2948935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑