Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,457	G→A	noncoding (71/77 nt)	metZ →	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,457	0	G	A	80.0%	12.9 / ‑4.7	10	noncoding (71/77 nt)	metZ	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (3/5);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACC  >  NC_000913/2947211‑2947647                                                                                                                                                                                                                                                       |                                                                                                                                                                                               aaCGAAGATAACAAACCTCCTCGTTTAATGAAAGGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAAGCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCACAAc                                                                                                                                                                                            <  1:191007/251‑1 (MQ=255)                 cgccgcGTTTAATGAAATGAGCAGTCTCTCCCTGACGCAAATTGTGCACAAAAAATATGCTTTAGTGATTTGTGTGTGTTCAAAAGCATGCCAAATCCGTGATCGTGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTAGAGTGCTCATCCACGGACGCGGTGTGGAGCAGCCGGTTAGCTCGTCAGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCACAACCAATTAAAATTtgatg                                                                                                                                                                            <  1:161982/251‑1 (MQ=37)                            atgaaatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATATCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCACAACCAATTAAAATTTGATGAAGTAAAGCAg                                                                                                                                                                 <  1:116182/251‑1 (MQ=255)                                         ccTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGAGCGGGGTAAAAAAAAGGGTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCTGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCACAACCAATTAAAATTtg                                                                                                                                                                               <  1:116943/224‑1 (MQ=255)                                                cTGACGCAAATTTTGCACAAAAAATAGGCTT‑AGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCACAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGagc                                                                                                                                            >  1:151861/1‑251 (MQ=255)                                                     gCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATCCCCACTCCGTGCTCCGGGTAAAAAAAAGGTTGCATCAAAACGCGAGCGGACTATAGTGCGCATACACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCACAACCAATTACAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                                        >  1:208884/1‑251 (MQ=25)                                                                                                          nccAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGCAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGATCAAATCCGGccccc                                                                                   >  1:52934/2‑251 (MQ=255)                                                                                                                     gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAATTGCTGAAACAAAGCAGTACGGGGACGCGGGGTGGAGCAGCCAGGTAGCTCGCAGGGCTCATAACCCGAAGGTCGGCGGTTCAAATcagacaaccgcacccaacc                                                                         >  1:155063/1‑232 (MQ=18)                                                                                                                            gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCACAACCAATTAAAATTTGATGAAGTAAAGc                                                                                                                                                                   >  1:12079/1‑153 (MQ=255)                                                                                                                                                                                           ggcagtcatgtaggttgtcggtataaTAACCCGAAGCTGATCGGTTCAAAACAGGCCCCCACAATCAACTAGAGTTCGGAGGAGTAAGGCAGTACGGTGACGGGGGGTGGAGCAGCCTTGTAGCTCGTCGGGCTCATAACCCGAAGGTGGGGGGTTGAAAAACGGCCCCCGCAACCAATCAAATTTGGTGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc  <  1:183558/226‑1 (MQ=9)                                                                                                                                                                                                                                                       |                                                                                                                                                                                               AACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACC  >  NC_000913/2947211‑2947647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑