Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 479,071 C→T G61S (GGC→AGC)  ylaC ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913479,0710CT91.7% 24.2 / ‑6.0 12G61S (GGC→AGC) ylaCputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (4/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGAACATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACCAGCGCGGCAGGTACAAAGCGCGTGTTGTACCATTCGCCGTTATAGCAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTG  >  NC_000913/478870‑479272
                                                                                                                                                                                                         |                                                                                                                                                                                                         
tATGAACCTCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACCAGCGCGGCAGGTACAAAGCGCGTGTTGTACCATTCGCCGTTATAGAAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCATTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc                                                                                                                                                           <  1:33308/250‑1 (MQ=255)
      cATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACCAGCGCGGCAGGTAAAAAGCGCGTGTTGTACCATTCGCCGTTATAGCAAACGCGGAAATCCAGCACGACGCTATCTTCATAGCGGCAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGATTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTAc                                                                                                                                                    >  1:35919/1‑251 (MQ=255)
                       gagTTCAAGATGGCTTCAACCAGCGCGGCAGGTACAAAGCGCGTGTTGTACCATGCGCCGTTATAGCAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGccn                                                                                                                                   <  1:51653/251‑2 (MQ=255)
                                   gCTTCAACAAGCGCGGAAGGTACAAAGCGCGAGTTGTACCATTCGCCGTTATAGCAAACGCGGAAATCAAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGACAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGAAAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCACCGGATGTTTACg                                                                                                                       >  1:165449/1‑251 (MQ=255)
                                             gcgcGGCAGGTACAAAGCGCGTGTTGTACCATTCGCCGTTATAGCAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAAGACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTAGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTg                                                                                                             <  1:194068/251‑1 (MQ=255)
                                                                 gtgtTGTACCATTCGCCGTTATAGCAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgnt                                                                                         <  1:168670/251‑1 (MQ=255)
                                                                                                 gAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCggg                                                                        <  1:142015/236‑1 (MQ=255)
                                                                                                                                       ngTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATCAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCAACGCCAGGTTGGCAAAAAAAGCAACATACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCAt                       >  1:22870/2‑247 (MQ=255)
                                                                                                                                          aGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGc                <  1:68333/251‑1 (MQ=255)
                                                                                                                                           gACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCTTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCaga                                                                >  1:77907/1‑202 (MQ=255)
                                                                                                                                                  aaaaaGAAGAAACCAGTAAGCAGGATAAATAATACAACCAGGAGCCAGACAGAGCTTGACAGCGTTTCGGACGGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACAGACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGGATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTgg        <  1:91567/251‑1 (MQ=255)
                                                                                                                                                         agaaACCATTAAGCAGGATAAATACTACAACCAGTAGACAGACAGCGCTTGACCGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAACAAAAGCCACGTACATACCGATAAACAGACCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGCATCTg  >  1:200185/1‑250 (MQ=255)
                                                                                                                                                                                                         |                                                                                                                                                                                                         
TATGAACATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACCAGCGCGGCAGGTACAAAGCGCGTGTTGTACCATTCGCCGTTATAGCAAACGCGGAAATCCAGCACGTCGATATCTTCATAGCGGTAGCGTGGGTAGACATCGAAAAAGAAGAAACCATTAAGCAGGATAAATAATACAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTG  >  NC_000913/478870‑479272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑