Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,933,521	G→A	V56V (GTC→GTT)	fucA ←	L‑fuculose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,933,521	0	G	A	100.0%	24.3 / NA	11	V56V (GTC→GTT)	fucA	L‑fuculose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

AAGTTCGCGTGTTCCAAAGGTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTATTGAATTAG  >  NC_000913/2933281‑2933751                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       aaGTTCGCGTGTTCCAAAGGTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATAGAAACTGCCGTTCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTGTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGTGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAAACAATATGCg                                                                                                                                                                                                                              <  1:221406/251‑1 (MQ=255)                                                                                                                              cATGATTGTGACAAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGcatcccat                                                                                                                                                                           >  1:167968/1‑177 (MQ=255)                                                                                                                                               ncgTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCa                                                                                  >  1:130959/2‑249 (MQ=255)                                                                                                                                                                gCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTAATGTTTACCGTCGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGCTAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCAtt                                                                >  1:122222/1‑250 (MQ=255)                                                                                                                                                                                     gAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTNCACNNNNNtt                                                                                                                                                     >  2:133455/1‑144 (MQ=255)                                                                                                                                                                                          nccATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAgg                                     >  1:132543/2‑251 (MQ=255)                                                                                                                                                                                                                           gNNTACCGTNNCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTATGCGTAATCAGCATCCCATCCTGATAACGTacac                                                                                                                                                            <  2:18265/99‑1 (MQ=255)                                                                                                                                                                                                                              tACCGTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTATTGAATTan  <  1:133455/250‑2 (MQ=255)                                                                                                                                                                                                                                  gTTGCCATCANNAAAAACAATNTGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCNTCCTGATAANNTANACTGANGNNNCNNNCTNTCCCCTGGTTCAGTCCCAGNCGNNTCATTTNCAGGCAAGTGTCAATAATCTGACGAg                                                                                    <  2:48405/164‑1 (MQ=255)                                                                                                                                                                                                                                  gTTGCCATCAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg                                                                                    >  1:48405/1‑164 (MQ=255)                                                                                                                                                                                                                                          cAATAAAAACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCg                                                                                                                       >  1:8473/1‑121 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       AAGTTCGCGTGTTCCAAAGGTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTATTGAATTAG  >  NC_000913/2933281‑2933751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑