Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,037	G→A	A244T (GCG→ACG)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,037	0	G	A	90.9%	23.3 / ‑6.1	11	A244T (GCG→ACG)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (8/2);  total (8/3)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAATGAACCCGGCCAACAAAGATCAGGTTTTACCATTGATCCTCACCGGCCCGAAAGAGAGCGCCGACTACTTCCGCGTACTGGACGAGTTTGTCGTGCATACGCT  >  NC_000913/2926815‑2927227                                                                                                                                                                                                                               |                                                                                                                                                                                               gtcgGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGCGCGCTGGAAGCGACGCTGAAAGGCGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGCACCGTCGTTTATCGCCGCTGAACCGCCTAACCCGCTGGTAAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCAt                                                                                                                                                                    >  1:213831/1‑251 (MQ=255)                                                        naGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGCGTTGCGCTATTTGCTGGGAAtt                                                                                                             >  1:173729/2‑251 (MQ=255)                                                        gAGCGAGGGAAGCGCCGATGAAAGGTGCTGCGGGCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCTATTATCGCCGCAGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAATCGTCGTGAAGCGTTTTTCCTTTTCGCTCACGGGATCATTGGCTGCCCTGGCGGGGGGGGTACGGCAGAAGAGTTGCTCTATTTGCTGTGAAtt                                                                                                             <  1:214751/251‑1 (MQ=255)                                                                        gATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATGGGTATGACCGAGCCGTCGATTATAGCCGATGAGCAGCCTAACCCGCTGGTCAACGAGTTGATCATCATGCCAGATATCGCAAGACGTCTGGCAACGTTTGTCCGTATCGCTCACGGTATCTTTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAATGAACACGGcc                                                                                              >  1:200930/1‑250 (MQ=255)                                                                                              nacGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGACGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGgtgt                                                                                                                                                  >  1:174044/2‑176 (MQ=255)                                                                                                             tACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCAGCATGCCAGATATCGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCAGTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGTGAATTTTAATGAACCCGGCCAACAAAGATCAGGTTTTACCATTGATCCTCACCGGcc                                                         <  1:139200/250‑1 (MQ=255)                                                                                                                                   ntATGACAGACCCGCCGATTATAGCAGCTGAACCGCCTAACACGCTGGTCAAAGAATTGATCATCATGCCAGATATCGAAAACCGTCTGGAAACGTTTGTCCGTATCGCTCAc                                                                                                                                                                            >  1:174674/2‑113 (MQ=255)                                                                                                                                                          gccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATAGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAATGAACCCGGCCAACAAAGATCAGGTTTTACCATTGATCCTCACCGGCCCGAAAGAGAGCGCCGACTACTTCCGCGTACTGGACGAGTTTGTCg            >  1:45246/1‑250 (MQ=255)                                                                                                                                                          gccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCAAGATATCGAAACACGTCTTGAGACGTTTGTCCGTATCGCTCACGGCATCATTATCTGCCCTGGCGGTGTGGGTGCGGCAGAAGAGTTGCGCTATTTGCTGGGAATTTTGATGAACGCGGCCAACAAAGATCAGGTTGTACCATTGATCCTCACCGGCCCGGACAAAAGCGCCGACTACTTCCGCGGACttgccgggtttttcg            >  1:45247/1‑236 (MQ=255)                                                                                                                                                                   ccGCCTAACCCGCTGGTCAACGAATTGATCATCATCCCCGACATCGAAAAACGCCTGCAAACGTTTGTCCGTATAGCTCACGGTACCATTATCTTCCCTGGCGGTGTGGGTACGGCAGACGCGCTGCTCTATTTGATGGGACTTTTACTGAACCCGGCCCACAAAGATCAAGTTTTACCCTTGATCCTCACCGGCCCGCAAGAGAGCGCCGACTACTTCCGCGTACTGGACGAGTTTGTAGTGCATACGCt  >  1:197412/1‑251 (MQ=255)                                                                                                                                                                                            tGATCATCATGCCAGATATCGAAAAACGTCTGGAAACGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAATGAACCCGGCCAACAAAGATCAGGTTTTACCATTGATCCTCACCGGCCCGAAAGAGAGCGCCGACTGCTTCCGCGTACTGGACGAGTTTGTCGTg          <  1:64265/218‑1 (MQ=255)                                                                                                                                                                                                                    agngTCNGNNANNGGTNCTCCGTATCGCGCACGGTCTCNNTAGCTTCCCNGGCGGTGTGCGTACGGCAGAACAGTTGCTCTATTTGCTGGGAATTTTAATGAANCCGGCCAACNNAGNCCAGTNTNNNCNNNTGNTCCTCACCGGCCCGAAAGANAGNNCCGACTNCTTCCGCGTTCTGTACGAGTTTGTCg            <  2:45246/189‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                               GTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAATGAACCCGGCCAACAAAGATCAGGTTTTACCATTGATCCTCACCGGCCCGAAAGAGAGCGCCGACTACTTCCGCGTACTGGACGAGTTTGTCGTGCATACGCT  >  NC_000913/2926815‑2927227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑