Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,918,257	G→A	R377C (CGC→TGC)	gudD ←	D‑glucarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,918,257	0	G	A	100.0%	13.2 / NA	7	R377C (CGC→TGC)	gudD	D‑glucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

GTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCGCTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGA  >  NC_000913/2918042‑2918460                                                                                                                                                                                                                        |                                                                                                                                                                                                            gTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCAATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTGTGATCTCAAACGGTTCTTTGGTCAGGCACTGATTGCCTTCCTGCCAAATCCAGTGCGTATc                                                                                                                                                                            <  1:202576/249‑1 (MQ=255)                           tGTTATCGAACGTCCAGCCAGGAATCAGGTACTGCATTCCCATCGCATCGGCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATCGGCTTTCAGCACTTGAGCCATATCGATTTCTACTCCCAGCCCCGGGTTTTCGGGCTCCTGTACCAGCCCCCCTTTTATCTCAAACGGTTCTCTGGTCAGGCACTTATTGCCTTCATGCCAAATCCAGTGCGTCTGAATAGc                                                                                                                                                                      <  1:76998/229‑1 (MQ=255)                                                                                 nngcnnNNNGCCNGTGTTTCTGATACAGCTCATGGGCTTTCATCACGTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCACTGATTGCCt                                                                                                                                                                                                   <  2:134929/144‑1 (MQ=255)                                                                                         aGCCCGTGTTTCTGATAAAGCTCATGGGCTTTCATCAATTGATCCATATCGATTTCTACACACAGACACGGTTTTTCTGGCACCTGTACCAGCCCGCCTTTGATCTCAAAAGGTTCTTTGGTCAGGCACTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGAAGTGGTTGTTAGAGTGTGAAcccc                                                                                   >  1:140657/1‑250 (MQ=255)                                                                                                                                                                         cACCTGTACCAGCCCGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCACTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACAGTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGa  <  1:117535/251‑1 (MQ=255)                                                                                                                                                                                      ccGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCACTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGANGTGnnnnnt                                                                                                 >  2:134755/1‑143 (MQ=255)                                                                                                                                                                                      ccGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCACTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATccn           <  1:134755/229‑2 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                            GTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTTGATCTCAAACGGTTCTTTGGTCAGGCGCTGATTGCCTTCCTGCCAAATCCAGTGCGTATCAATAGCAGTAATTTTACCCGGTGCAGCGGCGGCAACATGGGTAAACATCGCCAGGGAAATATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGA  >  NC_000913/2918042‑2918460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑