Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,773,451	C→T	T45I (ACC→ATC)	yfjW →	uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,773,451	0	C	T	100.0%	24.9 / NA	11	T45I (ACC→ATC)	yfjW	uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

ATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAACAATTTTATTTTTCACTGGAAACCGCATGGTACCTCATATCTGCTGTTGCAGTATTTATTGCATCTGTTTTTATACAGCATAGAATTAAAGCTTATTTAACATTATTAGCTATTACATGGATTGTACTAACAATAACAGATGTGGCGTTAAT  >  NC_000913/2773229‑2773661                                                                                                                                                                                                                               |                                                                                                                                                                                                                   aTCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGATAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCGTGGCATTTTTCTATATTTCCTCTATTTTTTTTCGTCTTTGTAGTGTTGTTCTCTTTCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTAt                                                                                                                                                                                        <  1:220974/251‑1 (MQ=255)                      cTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGAt                                                                                                                                                                   >  1:15641/1‑251 (MQ=255)                               cGATGATTTGTTGCGTATGATTAAAAATCAACCTTATAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGACAGATATTTAGCAACAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTTAATATTTTATCGCTGCCTTCACaaaa                                                                                                                                                                                                    >  1:148973/1‑209 (MQ=255)                                gatgatTTGTTGCGTATGATTACAAATCAACCTTCAAGACTTTATACATGGGTTTGCTCTGGCTTTTGTATAAGAAAGAGAAATTGTAAGAAAGATATTTAGCCAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTATCGCTGCCTTCACAACAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAAc                                                                                                                                                         >  1:92164/1‑251 (MQ=255)                                                                                                tttGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTCTGTAGTGGCGTTCTCTTTCAATATTGTATCGCTGTCTTCACAAAAGGTAATTGTTAGGAATGTTCAATGAAACGTGATTACATAAAACAATTTTATTTTTCACTGGAAACCGCATGTTACCTCATATCTGCTGTTGCAGTATTTATTGCa                                                                                           <  1:177615/249‑1 (MQ=255)                                                                                                                     gTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAAc                                                                                                                                                         >  1:109256/1‑166 (MQ=255)                                                                                                                                atTTATCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTGCCTCTATTTTTTTTCTTCTTGGTAGTGGCGTTCTCTTTCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAACAattttatt                                                                                                                                                <  1:48267/164‑1 (MQ=255)                                                                                                                                atTTAGCAAAAAAATTGACTTTACGANCTTGGCNNTTNTCTATATTTCCTCTATTTTNTTNNNTNNNTNTAGTGNCGNNCTCTTTCAANATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTNATTACATAANNCAattttatt                                                                                                                                                >  2:48267/1‑164 (MQ=255)                                                                                                                                      cAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATCTTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTATCTTTCACTATTTTATCGCTGCCTTCACACAAGGTAATTGTTATGAATGCTCAATGCAACTTGATTACATAAAACAATTTTATTTTTCACTGGCAACCGCATGGTACCTCATATCTGCTGTTGCAGTCTTTATTGCATCTGTTTTTATACAGCATAGAATTAAAGCTTATTTAACat                                                   >  1:89887/1‑251 (MQ=255)                                                                                                                                            aaTTGACTTTACGATCTTGGCATTTTTCTATAGTTCCTCTATTTTGTTTCTTCGTTGTAGGGGCGTTCTCTTGCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATGACATAAAACAATTTTATTTGTCACTGGAAACCGCATGGGACCTCATATCTGCTTTTGCAGTAGTTATTGCATCTGCTTGGATACAACATAGAATTAAAGCTTATTTAACATTATTAg                                             <  1:189802/251‑1 (MQ=255)                                                                                                                                                                                                                tctTTCAATATTTTATCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAACAATTTTATTTTTCACTGGAAACCGCATGGTACCTCATATCTGCTGTTGCAGTATTTATTGCATCTGTTTTTATACAGCATAGAATTAAAGCTTATTTAACATTATTAGCTATTACATGGATTGTACTAACAATAACAGATGTGGCGTTAAt  <  1:92316/226‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                   ATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTTACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTCTTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTTGATTACATAAAACAATTTTATTTTTCACTGGAAACCGCATGGTACCTCATATCTGCTGTTGCAGTATTTATTGCATCTGTTTTTATACAGCATAGAATTAAAGCTTATTTAACATTATTAGCTATTACATGGATTGTACTAACAATAACAGATGTGGCGTTAAT  >  NC_000913/2773229‑2773661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑