Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,691	G→A	noncoding (99/363 nt)	ssrA →	tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,691	0	G	A	91.7%	20.1 / ‑5.4	12	noncoding (99/363 nt)	ssrA	tmRNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/5);  minor base T (1/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAG  >  NC_000913/2755476‑2755874                                                                                                                                                                                                                        |                                                                                                                                                                                        ccATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGCGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGACTGATTCTGGATTCGACGGGATTTGCGAACCCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAACATAGTCGCCAACGCCACAAACTACGCTTTCGCAGCTTAATCAcc                                                                                                                                                             >  1:111936/1‑244 (MQ=255)               aaTCGAATAAAAATCATGCTACATGGGGGCCAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAAGCTGGTATACTTACGTTTACACATTGGGGCTGAGTCTTGATTCGACGGGAGTTTCGAAGCCCAATGTGCATGCCTAGGGGCGGTTTGCCTCGTAGAAAGCCGCAAAAAATAGGCGCAAACGGCAAAAACTTCGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTccc                                                                                                                                         <  1:92890/250‑1 (MQ=255)                                                                             tCATGGCGCTCATAAATCTGGTATACTTACCTTTACACTTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACAAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCACGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGcc                                                                          >  1:198587/1‑251 (MQ=255)                                                                                               tGGTATACTTACCTTTTCACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAAACCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACAAAAACGACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGc                                                         <  1:48043/250‑1 (MQ=255)                                                                                                                                         aCGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACTAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTgtccgtcc              >  1:73611/1‑251 (MQ=255)                                                                                                                                                   cGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAATCGCAAACGACACAAAATCCGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGCTCAAGAGAGGGCAAACCCAAAAGAGATCGCGTGGCAGCCCCGCCTGGGGGTGAAGCGTTAAAACTTAATc                                           >  1:102561/1‑212 (MQ=255)                                                                                                                                                       aCCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACAAAAACTAAGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCCCTCTCCCTAGCCTCCGCTCTTAGGACNGGGNNNNngannnnnn                                                                                                     >  2:136068/1‑144 (MQ=255)                                                                                                                                                                                  ggCCTCGTAAAAAGCCGCAAAAAATAGTCGCAACCGACAAAAACTACGCTTTAGCCGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTCGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTCGTTTGTTAGTGGCGTGGCCGTCCGCAGcc        >  1:86178/1‑215 (MQ=255)                                                                                                                                                                                                  gCAAAAAATAGTCGCAAACGACAAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAATCGTGAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGGCCGTCCGCAGCTGGCAAg  <  1:37552/206‑1 (MQ=255)                                                                                                                                                                                                               gCAAACGACAAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGANCTCTCNCNCNNGANNCTCCGctc                                                                                                                             <  2:140989/70‑1 (MQ=255)                                                                                                                                                                                                               gCAAACGACAAAAACTACGCTTTAGCAGCTTAATAACCTACTTAGAGCACTCTATCCATAGCCTCCGctc                                                                                                                             >  1:140989/1‑70 (MQ=255)                                                                                                                                                                                                                  aaCGACAAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTgggn                                                                <  1:53923/128‑2 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                        CCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAG  >  NC_000913/2755476‑2755874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑