Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	465,330	C→T	S73N (AGC→AAC)	queC ←	7‑cyano‑7‑deazaguanine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	465,330	0	C	T	100.0%	14.1 / NA	7	S73N (AGC→AAC)	queC	7‑cyano‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/5);  total (2/5)

CGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCT  >  NC_000913/465095‑465513                                                                                                                                                                                                                                            |                                                                                                                                                                                        cGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCt                                                                                                                                                                          >  1:48733/1‑251 (MQ=255)                                                cgcgGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGAGACCATCGGCTGCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCACCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAg                                                                                                                            <  1:164033/250‑1 (MQ=255)                                                                        aaTCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTcg                                                                                                            <  1:166086/242‑1 (MQ=255)                                                                                                                       tACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGACCTGGGCCAAACGTATTCGGGATACCATCGGCTTCAGGTTCATCATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCTCGTTGAGCAGGGAGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGCTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCCCGCAATg                                                    >  1:122677/1‑251 (MQ=255)                                                                                                                                                                  cTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGTCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCTCTTTGAGCAGTGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGGGCCACGTCGATTTCTGCGCGATg                                                                                  <  1:82401/178‑1 (MQ=255)                                                                                                                                                                         aaaCGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATccn  <  1:53241/251‑2 (MQ=255)                                                                                                                                                                                                cTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGTTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATgcgcgn                                                                                                                                      <  1:24796/96‑2 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                        CGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCT  >  NC_000913/465095‑465513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑