Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,4570AG66.7% 15.5 / 10.6 19I46I (ATT→ATC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (5/7);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-02
Rejected as consensus: Frequency below/above cutoff threshold.

AGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTT  >  NC_000913/579223‑579683
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
aGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCtg                                                                                                                                                                                                                    >  1:69631/1‑251 (MQ=255)
                        ggTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAAtgtg                                                                                                                                                                                            <  1:45331/251‑1 (MQ=255)
                              ntAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGAcgt                                                                                                                                                                                      >  1:131074/2‑249 (MQ=255)
                                aaTATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTtg                                                                                                                                                                                    <  1:47705/251‑1 (MQ=255)
                                                                            ccTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACTTTTCTATAAGATGCGGGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACTATGTGAATATGATCTTTGGCTAGATATTAAATATAATGTGAGATGGTGTGACGGTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCttt                                                                                                                                        <  1:93794/251‑1 (MQ=255)
                                                                               gCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGCATTTACCATTTACCACCTTTTTAAGTCCTTTTATTAACACGGAGTTATCGTTTTCTAACACGCTGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGCATAAAACAATTCACAGTCTAAATCTTTTCg                                                                                                                                     >  1:138374/1‑251 (MQ=255)
                                                                               gCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTAACTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAAACTTTTTAAGTACTTTTATTACAACGGTGTTATCGTTTTTTAACACAATGTGCATATTATCTGTGGCTAGATAGAAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATACAATAATTCACAGTTTAAATATTTTCg                                                                                                                                     >  1:216838/1‑251 (MQ=255)
                                                                                      cAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATGTGTGGCTAGATAGTAAATATAATGTGAAACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGa                                                                                                                              <  1:221051/251‑1 (MQ=255)
                                                                                               cnntgttntatggataTGTGATTTCTCTTGATNTCAACCTATCATAGGACGTTTCTATAAGATTNGNTTTGCTTGAGAAGTTAACATTTACATCCTTTTTAAGTCCTTTTATTAANACGGTGTTATNGTTNNCTAANANGNTGTTAAGATTATCTGTGGCTAGATAGTAANTATAACGTGAGACGTTGTGACGGNNTANNNCANNATaaa                                                                                                                                                              <  2:164546/195‑1 (MQ=255)
                                                                                                            aaaTGTTATTTCTCTTGATGTCATCCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGGCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTGCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATgg                                                                                                        <  1:150922/251‑1 (MQ=255)
                                                                                                                                      tATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATGTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTATATATAATGTGAGACGTTGTGACGTTTTAGTTCATAATAATACAATTCACATTCTAAAGCTTTCCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCtn                                                                               <  1:26759/250‑2 (MQ=255)
                                                                                                                                         cANNNNNCGTNTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGAcgt                                                                                                                                                                                      <  2:131074/144‑3 (MQ=255)
                                                                                                                                                       ataAGATGCGTATTTCTTGAGAATTTAACATTTACATCCTTTTTAAGTCCTTTTATTAACACGGTGTTAGCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACAGTGTGACGTTTTAGTGCAGAATAAAACAATTCACAGTTTAAATCTTTACGCGCTTGAGCGAATATTTCGTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGTTACGAgg                                                                <  1:152209/248‑1 (MQ=255)
                                                                                                                                                                                  aCATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTgg                                                                                        <  1:76246/197‑1 (MQ=255)
                                                                                                                                                                                                     gTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGCGATACGAGGGCGCGTAGTTTg                                                    >  1:57976/1‑214 (MQ=255)
                                                                                                                                                                                                       ccTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACNNTTTAGtnn                                                                                                                                                                      >  2:5756/1‑96 (MQ=255)
                                                                                                                                                                                                             attaACCCNGNNNTNNCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAANTATAATGTGAGACGTNGTNACGt                                                                                                                                                                              >  2:153082/1‑84 (MQ=255)
                                                                                                                                                                                                             attaACACTGTTTGATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGt                                                                                                                                                                              <  1:153082/84‑1 (MQ=255)
                                                                                                                                                                                                                  cacGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttgtt  >  1:30656/1‑251 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
AGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTT  >  NC_000913/579223‑579683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑