Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,3850AG62.5% 8.3 / 10.8 17N70N (AAT→AAC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (5/5);  total (7/10)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGG  >  NC_000913/579152‑579621
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
gTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATATTAATATCGTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGAGTTTCCCGGTATTGCTTAAATGTGATTTTTCTTTATTGCAACCTATCAGAGGACGTTTCTATAAGATGCGTATTTCGTGAGAATTTAAc                                                                                                                                                                                                                             <  1:121190/251‑1 (MQ=25)
 tGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGACAGATTTGTGTAGTGTTATGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATCTTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAAc                                                                                                                                                                                                                             >  1:103238/1‑250 (MQ=255)
                                       ggAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTACATAGTAATGAATTATCAAAGGGATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGTTTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGTTGAGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGtt                                                                                                                                                                                      >  1:10846/1‑251 (MQ=255)
                                                                       aGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCtg                                                                                                                                                      >  1:69631/1‑251 (MQ=255)
                                                                                               ggTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAAtgtg                                                                                                                              <  1:45331/251‑1 (MQ=255)
                                                                                                     ntAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGAcgt                                                                                                                        >  1:131074/2‑249 (MQ=255)
                                                                                                       aaTATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTtg                                                                                                                      <  1:47705/251‑1 (MQ=255)
                                                                                                                                                   ccTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACTTTTCTATAAGATGCGGGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACTATGTGAATATGATCTTTGGCTAGATATTAAATATAATGTGAGATGGTGTGACGGTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCttt                                                                          <  1:93794/251‑1 (MQ=255)
                                                                                                                                                      gCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGCATTTACCATTTACCACCTTTTTAAGTCCTTTTATTAACACGGAGTTATCGTTTTCTAACACGCTGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGCATAAAACAATTCACAGTCTAAATCTTTTCg                                                                       >  1:138374/1‑251 (MQ=255)
                                                                                                                                                      gCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTAACTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAAACTTTTTAAGTACTTTTATTACAACGGTGTTATCGTTTTTTAACACAATGTGCATATTATCTGTGGCTAGATAGAAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATACAATAATTCACAGTTTAAATATTTTCg                                                                       >  1:216838/1‑251 (MQ=255)
                                                                                                                                                             cAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATGTGTGGCTAGATAGTAAATATAATGTGAAACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGa                                                                <  1:221051/251‑1 (MQ=255)
                                                                                                                                                                      cnntgttntatggataTGTGATTTCTCTTGATNTCAACCTATCATAGGACGTTTCTATAAGATTNGNTTTGCTTGAGAAGTTAACATTTACATCCTTTTTAAGTCCTTTTATTAANACGGTGTTATNGTTNNCTAANANGNTGTTAAGATTATCTGTGGCTAGATAGTAANTATAACGTGAGACGTTGTGACGGNNTANNNCANNATaaa                                                                                                <  2:164546/195‑1 (MQ=255)
                                                                                                                                                                                   aaaTGTTATTTCTCTTGATGTCATCCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGGCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTGCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATgg                                          <  1:150922/251‑1 (MQ=255)
                                                                                                                                                                                                            cTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAaca                                                                                                                                                                        >  1:187185/1‑100 (MQ=255)
                                                                                                                                                                                                             tATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATGTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTATATATAATGTGAGACGTTGTGACGTTTTAGTTCATAATAATACAATTCACATTCTAAAGCTTTCCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCtn                 <  1:26759/250‑2 (MQ=255)
                                                                                                                                                                                                                cANNNNNCGTNTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGAcgt                                                                                                                        <  2:131074/144‑3 (MQ=255)
                                                                                                                                                                                                                              ataAGATGCGTATTTCTTGAGAATTTAACATTTACATCCTTTTTAAGTCCTTTTATTAACACGGTGTTAGCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACAGTGTGACGTTTTAGTGCAGAATAAAACAATTCACAGTTTAAATCTTTACGCGCTTGAGCGAATATTTCGTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGTTACGAgg  <  1:152209/248‑1 (MQ=255)
                                                                                                                                                                                                                                         aTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGttt                                                                                                                                                                               <  1:17534/64‑1 (MQ=255)
                                                                                                                                                                                                                                         aTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGttt                                                                                                                                                                               >  2:17534/1‑64 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
GTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGG  >  NC_000913/579152‑579621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑