Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,887	Δ1 bp	coding (678/1221 nt)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,887	0	C	.	100.0%	34.1 / NA	10	coding (678/1221 nt)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/6);  total (4/6)

ATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGG  >  NC_000913/1667652‑1668116                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      aTGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGCCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTATGCAAGCTCAAGAATACTGTCCACGCTGGCGACGGTTTCGAGGCAGCCGTGATTCACGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGCCTACTGCTGCCTGCGTGTAGCAGATGACCACCGGTAATGCCGCC‑ACCCCCACGTTGTGAt                                                                                                                                                                                                                       >  1:191815/1‑251 (MQ=255)           acacAATGAGTCCTCGGTTAACGGTTTTCCATTTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATTCTGTCCACGCTGGCGATGGTTTCGTTGTAGCCGTGACTCCCGCTATAACAGCGTTTCCCATTCGTGTCGACCTGTGTGTTGCCGAGTTCCTCGAGACGACTTCTGCCTGCGTGTAGCGTATGACCATCGGTAATGACGCC‑GCCCCCACTTTGTGATCGATAaccac                                                                                                                                                                                                             <  1:98253/251‑1 (MQ=255)                                  gTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGTAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATCTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCcgc                                                                                                                                                                                      >  1:216009/1‑251 (MQ=255)                                                      cTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGACCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTTAATCACATcgcgcgn                                                                                                                                                                                           <  1:136855/226‑2 (MQ=255)                                                                   tAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCAGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTc                                                                                                                                                      <  1:172197/250‑1 (MQ=255)                                                                     ngACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGt                                                                                                                                                                                    >  1:135565/2‑218 (MQ=255)                                                                                        naaGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATc                                                                                                                                                                                                                      >  1:19094/2‑165 (MQ=255)                                                                                                  gTCCACGCTGGCGATGTTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTGCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCTCCCCGCGTGAGGCAGCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAAcc                                                                                                                        <  1:214871/249‑1 (MQ=255)                                                                                                                                               aCNNNNNTTCNCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGt                                                                                                                                                                                    <  2:135565/144‑1 (MQ=255)                                                                                                                                                                                                                      gATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATAGCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATgg  <  1:123484/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      ATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGG  >  NC_000913/1667652‑1668116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑