Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,388	Δ1 bp	coding (684/924 nt)	ydcI ←	DNA‑binding transcriptional dual regulator YdcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,388	0	C	.	100.0%	40.3 / NA	11	coding (684/924 nt)	ydcI	DNA‑binding transcriptional dual regulator YdcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/7);  total (4/7)

CCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCG  >  NC_000913/1494144‑1494619                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        ccgttaaGAACGTCATTGATTTTCTAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCCCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCATCGTGGCATGACTCATGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACCCGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCACACAGCGTTTCGATACAACC‑GAAGGaa                                                                                                                                                                                                                                  <  1:197452/245‑1 (MQ=255)                        cGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATGCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCAc                                                                                                                                                                                                            <  1:180674/250‑1 (MQ=255)                                         caACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTACAATCGGTTCGCCTGCACAATGTCCCGGAACCGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACACACTACACGAACTCGTATTCACCCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAAAC‑GAAGGAATTTTACAGCCCAGGCTCTGCACTAATGCATCTGAATGCtg                                                                                                                                                                                          >  1:155987/1‑251 (MQ=255)                                                       aagaGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACGCGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAATGAAGTTTACAGCCCTGTCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGc                                                                                                                                                                            <  1:199851/251‑1 (MQ=255)                                                                                       ccAAGCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAAGCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGt                                                                                                                                                                    <  1:154058/227‑1 (MQ=255)                                                                                        caagcGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTGTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAg                                                                                                                                                                               <  1:82645/211‑1 (MQ=255)                                                                                                                                                        ntcTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACg                                                                            >  1:130912/2‑250 (MQ=255)                                                                                                                                                                          aGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                         <  1:149924/251‑1 (MQ=255)                                                                                                                                                                               cAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGCCAGCGTTTCGATACAACA‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATCCAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCg                                                                                                          >  1:159915/1‑197 (MQ=255)                                                                                                                                                                               cAAACCACACGTAATCGTANTCAACCGTAAGTTGACGAGATAGCGAAGCAGNCNGCGTTGCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAANGCATCTGAATNCTGNNGTGGNGNANTGCCTTCTGGTGATACAACGACCGGCCATNCCAGCACCCGGCTTAGCGTTACGNNCTNNNGANNTAGCg                                                                                                          <  2:159915/197‑1 (MQ=255)                                                                                                                                                                                                                                 gACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCg  >  1:78725/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        CCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCG  >  NC_000913/1494144‑1494619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑