Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,5400TC70.6% 52.7 / 15.0 35N19D (AAC→GAC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base T (2/8);  new base C (10/14);  total (12/23)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGG  >  NC_000913/579305‑579768
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    
ttAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGc                                                                                                                                                                                                                         <  1:24259/249‑1 (MQ=255)
   gCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTg                                                                                                                                                                                                                    >  1:13911/1‑251 (MQ=255)
                           aaTGTNNTTNNNCTNNATTTCAACCTATCATAGGACGTTNCTATAAGATGCGTATTTCTTGCGGATTTANCNTNTACANNCTTNTTAAGTCCTTNTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATCTTATCTGTNGNTAGATAGTAAATATAATGTGAGACATTGTGANGTTTTAGTTCAGAATAAAACACTTNCCAGNNTanannnn                                                                                                                                                                                                                              >  2:237405/1‑213 (MQ=38)
                                                  ccTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTCGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGa                                                                                                                                                                                                               <  1:140068/209‑1 (MQ=255)
                                                                     ataAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTGTAAATCGTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCATCCTGAGCCATTGGTAAAACCTTCCATGGGATACGAggg                                                                                                                                                    <  1:237405/249‑1 (MQ=255)
                                                                        aGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACt                                                                                                                                                                                                                      <  1:17711/180‑1 (MQ=255)
                                                                              gTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCAGTGGTAAAACCTTCCATGTGAGACGAg                                                                                                                                                      <  1:257397/238‑1 (MQ=255)
                                                                                       gagaATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCgn                                                                                                                                <  1:96823/251‑2 (MQ=255)
                                                                                              naaNATTNANNANNTTTNTAAGTCCTTTTATTAACACGGNNTTATCGTTTNCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACANTGTGACGTTNNAGNTCAGANTNNNANNNTTNACAGTTTAAATCGTTTCGCNCTNNATCGAANATTTCTTTAAAGATGGCAAGCTGAGc                                                                                                                                                                                   <  2:75201/192‑1 (MQ=25)
                                                                                                    ttACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTAGCGTGTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAAGTCACAGTCTAAATCTGTTCGCACTTGATCGAATATTTCGTTAAAAAGGGCATCCTAAGCCAGTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCg                                                                                                                        <  1:49797/246‑1 (MQ=255)
                                                                                                                      cTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGACTATTATCTGTGGCTAGATAGTAAATAACATGTGAGACGTTGCGACGTTTTAGTTAAGAATAAAACAATTCACAGCCTAAATCTTTTCGCACTTGATCGAATATTTCCTTAAAAATGGCAACCTGAGCCATTGGTaaaa                                                                                                                                                                        >  1:327918/1‑180 (MQ=255)
                                                                                                                        tttATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGATACATTGTGACATTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCGTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCttt                                                                                                 <  1:3233/249‑1 (MQ=255)
                                                                                                                                cacGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGCGAGACGTTGTGACGTTTTAGATCAGAGTAAAACAATTCACAGTCTAAATCTTTTCGCACCAGATCCAATATGTCTTTAAAAATGACAACCTGCGCCATTGGTa                                                                                                                                                                           >  1:180205/1‑167 (MQ=255)
                                                                                                                                 acGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTttgttg                                                                                      <  1:7802/251‑1 (MQ=255)
                                                                                                                                                tcgaaCACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTGTTCGCACTTGATCGAATTTTTCTTTAAAAATGGCGACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAgg                                                                                                                                                     <  1:246872/170‑1 (MQ=255)
                                                                                                                                                       ngaTGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCAt                                                                                                                                      >  1:196960/3‑181 (MQ=255)
                                                                                                                                                           gtgAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCtttt                                                                                                                                                                                                                            <  1:5946/91‑1 (MQ=255)
                                                                                                                                                           gtgAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCtnnt                                                                                                                                                                                                                            >  2:5946/1‑91 (MQ=255)
                                                                                                                                                             gAATANNATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACt                                                                                                                                                                                                                      <  2:12984/95‑1 (MQ=255)
                                                                                                                                                                           gCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGACGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGt                                             >  1:138414/1‑250 (MQ=255)
                                                                                                                                                                                   gTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAAt                                                                                                                                                                                               >  1:210515/1‑96 (MQ=255)
                                                                                                                                                                                         ataATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAAc                               <  1:153016/250‑1 (MQ=255)
                                                                                                                                                                                         ataATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTgg                                                                                                             <  1:247868/172‑1 (MQ=255)
                                                                                                                                                                                            aTNNNNNACGNTGTGACGTTTTAGTTCATAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTGAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCAt                                                                                                                                      <  2:196960/144‑1 (MQ=37)
                                                                                                                                                                                                gagaCGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTNc                        <  1:254294/250‑1 (MQ=255)
                                                                                                                                                                                                  gacgttGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCgttt                                                                                                                     >  1:50815/5‑152 (MQ=255)
                                                                                                                                                                                                        gnnACGTTTNNGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAAc                                                                                                                                                                       <  2:13911/99‑1 (MQ=255)
                                                                                                                                                                                                         tgACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACGTGAGCGAATATTTCGTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCg                                                                                                                                 <  1:280320/136‑1 (MQ=255)
                                                                                                                                                                                                                     tCAGANTAAAACAATTCACAGTCTAAATCTTTTCGCANTNGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTNCCATGTGATANGAGNNCGCGNANTNTGCATTATCGTTTTTATCGTTTCAATCTGNTCTGACCTCCTTGTGTTTTGTTGNNGANNNATNNCAAat                                                                      <  2:242929/183‑1 (MQ=255)
                                                                                                                                                                                                                     tCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTACAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGCGCTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAgg  >  1:141256/1‑251 (MQ=255)
                                                                                                                                                                                                                     tCAGAATAAAACAATTCACAGTTTAAATCGTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGGGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTGTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAg   <  1:232151/250‑1 (MQ=255)
                                                                                                                                                                                                                     tCAGAATAAAACAATTCACAGTCTAAATCTTTTCGTAATTGATCGAATATTTCTTTAACAACAACAACCTGCGCCATTCGTAAAACCTTACATGAGATAAGAGGGCGCGTAGTTTGCATTATCGTTTTTCTCGTTTCAACGTGATCTGACATCCTTGTGTTTTGTTGATGATTTATGtaaact                                                                      >  1:242929/1‑178 (MQ=255)
                                                                                                                                                                                                                     tCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCGGCTGGCATTCTGGAg   <  1:110152/250‑1 (MQ=255)
                                                                                                                                                                                                                      cAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGtttt                                                        >  1:258300/1‑196 (MQ=255)
                                                                                                                                                                                                                             aaaCAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGATCCATTGGTAAAACCTTCCATGTTATACGAGGGCGCGTAGTTTGCATTATCGTTTTGATCGCTTCAATCTGGTCGGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAg                                                                 <  1:209527/180‑1 (MQ=255)
                                                                                                                                                                                                                               aCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAACCATGGCACCATGAGCCATTGGTAAA‑CCTTCCACGTGATAAGAGGGCGCGTAGTTTGCATTCTCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATAATAGGCATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGc            >  1:312077/1‑230 (MQ=255)
                                                                                                                                                                                                                                cAATTNACAGTNTNNCTCTTTTCGCCCTTGCTCGNATATTTCTTTAAAAANGGCANCCTGANctnt                                                                                                                                                                                >  2:133270/1‑63 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    
TTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGG  >  NC_000913/579305‑579768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑