Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,5020AG57.1% 12.9 / 27.6 28N31N (AAT→AAC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base A (4/8);  new base G (6/10);  total (10/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGC  >  NC_000913/579274‑579746
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
gATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTACTGCTGTAATGTGATTTCTCTTAATTTAAAACTATCATAGGAAGTTTCTATACGATGCGTATTTCTTGAGAATTTAACATTTAAAACCTTTTAAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGATAGTTAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAAt                                                                                                                                                                                                                                >  1:239562/1‑251 (MQ=255)
          aTCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGGGAATTTAACATTTAAAACATTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTat                                                                                                                                                                                                                                        >  1:178946/1‑232 (MQ=255)
                              tttAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATtgtn                                                                                                                                                                                                                                                 <  1:84893/204‑2 (MQ=255)
                               ttAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGc                                                                                                                                                                                                   <  1:24259/249‑1 (MQ=255)
                                  gCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTg                                                                                                                                                                                              >  1:13911/1‑251 (MQ=255)
                                                          aaTGTNNTTNNNCTNNATTTCAACCTATCATAGGACGTTNCTATAAGATGCGTATTTCTTGCGGATTTANCNTNTACANNCTTNTTAAGTCCTTNTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATCTTATCTGTNGNTAGATAGTAAATATAATGTGAGACATTGTGANGTTTTAGTTCAGAATAAAACACTTNCCAGNNTanannnn                                                                                                                                                                                                        >  2:237405/1‑213 (MQ=38)
                                                                                 ccTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTCGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGa                                                                                                                                                                                         <  1:140068/209‑1 (MQ=255)
                                                                                                    ataAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTGTAAATCGTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCATCCTGAGCCATTGGTAAAACCTTCCATGGGATACGAggg                                                                                                                              <  1:237405/249‑1 (MQ=255)
                                                                                                       aGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACt                                                                                                                                                                                                <  1:17711/180‑1 (MQ=255)
                                                                                                             gTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCAGTGGTAAAACCTTCCATGTGAGACGAg                                                                                                                                <  1:257397/238‑1 (MQ=255)
                                                                                                                      gagaATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCgn                                                                                                          <  1:96823/251‑2 (MQ=255)
                                                                                                                             naaNATTNANNANNTTTNTAAGTCCTTTTATTAACACGGNNTTATCGTTTNCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACANTGTGACGTTNNAGNTCAGANTNNNANNNTTNACAGTTTAAATCGTTTCGCNCTNNATCGAANATTTCTTTAAAGATGGCAAGCTGAGc                                                                                                                                                             <  2:75201/192‑1 (MQ=25)
                                                                                                                                   ttACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTAGCGTGTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAAGTCACAGTCTAAATCTGTTCGCACTTGATCGAATATTTCGTTAAAAAGGGCATCCTAAGCCAGTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCg                                                                                                  <  1:49797/246‑1 (MQ=255)
                                                                                                                                                     cTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGACTATTATCTGTGGCTAGATAGTAAATAACATGTGAGACGTTGCGACGTTTTAGTTAAGAATAAAACAATTCACAGCCTAAATCTTTTCGCACTTGATCGAATATTTCCTTAAAAATGGCAACCTGAGCCATTGGTaaaa                                                                                                                                                  >  1:327918/1‑180 (MQ=255)
                                                                                                                                                       tttATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGATACATTGTGACATTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCGTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCttt                                                                           <  1:3233/249‑1 (MQ=255)
                                                                                                                                                               cacGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGCGAGACGTTGTGACGTTTTAGATCAGAGTAAAACAATTCACAGTCTAAATCTTTTCGCACCAGATCCAATATGTCTTTAAAAATGACAACCTGCGCCATTGGTa                                                                                                                                                     >  1:180205/1‑167 (MQ=255)
                                                                                                                                                                acGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTttgttg                                                                <  1:7802/251‑1 (MQ=255)
                                                                                                                                                                               tcgaaCACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTGTTCGCACTTGATCGAATTTTTCTTTAAAAATGGCGACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAgg                                                                                                                               <  1:246872/170‑1 (MQ=255)
                                                                                                                                                                                      ngaTGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCAt                                                                                                                >  1:196960/3‑181 (MQ=255)
                                                                                                                                                                                          gtgAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCtttt                                                                                                                                                                                                      <  1:5946/91‑1 (MQ=255)
                                                                                                                                                                                          gtgAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCtnnt                                                                                                                                                                                                      >  2:5946/1‑91 (MQ=255)
                                                                                                                                                                                            gAATANNATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACt                                                                                                                                                                                                <  2:12984/95‑1 (MQ=255)
                                                                                                                                                                                                          gCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGACGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGt                       >  1:138414/1‑250 (MQ=255)
                                                                                                                                                                                                                  gTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAAt                                                                                                                                                                         >  1:210515/1‑96 (MQ=255)
                                                                                                                                                                                                                        ataATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAAc         <  1:153016/250‑1 (MQ=255)
                                                                                                                                                                                                                        ataATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTgg                                                                                       <  1:247868/172‑1 (MQ=255)
                                                                                                                                                                                                                           aTNNNNNACGNTGTGACGTTTTAGTTCATAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTGAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCAt                                                                                                                <  2:196960/144‑1 (MQ=37)
                                                                                                                                                                                                                               gagaCGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTNc  <  1:254294/250‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
GATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGC  >  NC_000913/579274‑579746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑