Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,666,074	IS1 (+) +9 bp	coding (640‑648/1281 nt)	csiE →	stationary phase‑inducible protein CsiE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	279163 =	NA (NA)	18 (1.270)	15/310	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2666074 =	0 (0.000)					coding (640/1281 nt)	csiE	stationary phase‑inducible protein CsiE
*	?	NC_000913	= 291401	NA (NA)	8 (0.570)	6/308	0.6	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2666082	0 (0.000)					coding (648/1281 nt)	csiE	stationary phase‑inducible protein CsiE

GCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAATTACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACG  >  NC_000913/2665825‑2666315                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  tcaccctttgacgtggtgatatggatgacggatggctggccgctgtatgaatcccgcctgaagggaaagctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCt                                                                                                                                                                                                                                                  >  1:131900‑M2/250‑251 (MQ=255)                     atggatgacggatggctggccgctgtatgaatcccgcctgaagggaaagctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataaggtggagtcattaccCTTTTGTCCATGTTGCGGGTTc                                                                                                                                                                                                                              <  1:105732‑M2/22‑1 (MQ=255)                             cggatggctggccgctgtatgaatcccgcctgaagggaaagctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatcttaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggttgagcagcataacaaagtcatcgttcattatctgaacataaaacactatcaataagttggagtcatttccCtt                                                                                                                                                                                                                                                 <  1:297102‑M2/3‑1 (MQ=255)                              gCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGACTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAATTACGCCTCAGTTTAATCCACTCACACGTCGCTGGGCGGAATCCTGCCTTGAATTACAGGAAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCggtgatgctgccaacttactg                                                                                                                                                                                                                      <  1:228426‑M2/250‑22 (MQ=255)                                                       atTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAATTACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTGTGGCTCTACTTTTTTCCggtgatgctgccaacttactgatttagtgtatgatggtgtttttgag                                                                                                                                                                                            >  1:136997‑M2/1‑204 (MQ=255)                                                         tCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAATTACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTGCTTTTTTCCggtgatgcttccaacttactgatttagtgtatgatggtgtttttgagg                                                                                                                                                                                           >  1:24700‑M2/1‑202 (MQ=255)                                                                ggaaagctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgtgcattatctgaacataaaacactatcaataagtttgagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCg                                                                                                                                                                                   <  1:268427‑M2/65‑1 (MQ=255)                                                                     gctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaactcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCgg                                                                                                                                                                                                                                  >  1:124225‑M2/182‑199 (MQ=255)                                                                                                gcaCCACGCTGGAATTACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggt                                                                                                                                                                                          <  1:127347‑M2/212‑50 (MQ=255)                                                                                                     gaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTTCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAg                                                                                                                                              <  1:283689‑M2/102‑1 (MQ=255)                                                                                                                      ctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgcacatacaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGGCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGttt                                                                                                                             >  1:11416‑M2/133‑251 (MQ=255)                                                                                                                             tgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCatn                                                                                                                                                                                                      <  1:262371‑M2/46‑2 (MQ=255)                                                                                                                                 gCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaa                                                                                                                   <  1:161935‑M2/250‑121 (MQ=255)                                                                                                                                       cctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGCTGAACAGGGGt                                                                                                            >  1:229063‑M2/116‑251 (MQ=255)                                                                                                                                                        ggaagtcgctgtcgttctcaaaatcggtggagcagcatgacaaagtcatcgggcattatcagaacataaaacaatatcaataagttggcgtcattaccCTTTTTTCCCTGTTGAGGGTTACCGATcc                                                                                                                                                                                                                       >  1:187933‑M2/99‑127 (MQ=255)                                                                                                                                                                  gtcgttctcaaaatcggtggagcagccagacaaacccaaagggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTTCATGTTGCggg                                                                                                                                                                                                                                 >  1:271837‑M2/89‑107 (MQ=255)                                                                                                                                                                                cggtggagcagcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTGTATACCCACCTCGCCCAgg                                                                           <  1:90211‑M2/169‑1 (MQ=255)                                                                                                                                                                                 ngaAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcactgccggacatcagcgctatctctgctctcactgccgtaaaac                                                                   <  1:85359‑M2/249‑169 (MQ=255)                                                                                                                                                                                     gagcatcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGc                                                                >  1:256720‑M2/70‑249 (MQ=255)                                                                                                                                                                                           cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcactgccggacatcagcgctatctctgctctcactgccgtaaaacatggcaactgc                                                        >  1:104604‑M2/1‑72 (MQ=255)                                                                                                                                                                                                          nggcattatctgaacataaaacactatcaataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGAGGGATGCGCCTCAGCGGGCCAGACAATTGCGTCAGTCTCTCAAACGTCTGGTACACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                          >  1:29394‑M2/49‑250 (MQ=255)                                                                                                                                                                                                            gcattatctgaacatacaacactataaatcagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAACCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCG‑TTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAg                                                                            >  1:98282‑M2/47‑213 (MQ=255)                                                                                                                                                                                                            gcattatctgaacataaaacactatccataagttggagtcattaccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGAGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGat                                        >  1:152160‑M2/47‑250 (MQ=255)                                                                                                                                                                                                                                                 gtcatttccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGAGCAGCTTTATACCCACCTCGCCCAGGCGGTAAATCGCAGTTTGTTGGCCATCGGTATTGAGAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACg  <  1:301702‑M2/242‑1 (MQ=255)                                                                                                                                                                                                                                                   gtcacTACTTTTTTCCggtgatgctgccaacttactgatttcgtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtcactcatgttcagctactgacggggtggtgcgtaacggcaaaagcactgccggacctcagcgctatctctgctctcactgccgtaaaacatggcaactgcagttcacttaca                                            <  1:298358‑M2/205‑192 (MQ=255)                                                                                                                                                                                                                                                        ccctTTTTTCCATGTTGCGGGTTCCCNATCCATNNCGNGATg                                                                                                                                                                                                            >  2:71905/3‑42 (MQ=255)                                                                                                                                                                                                                                                        ccCTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATg                                                                                                                                                                                                            <  1:71905‑M2/40‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  GCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAATTACGCCTCAGTTTAATCCGCTCCAACGTCGCTGGGCGGAATCCTGCCTTGAATTTCAGGTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACG  >  NC_000913/2665825‑2666315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.