Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,919,454	G→A	V649I (GTC→ATC)	letB →	lipophilic envelope spanning tunnel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,919,454	0	G	A	100.0%	31.3 / NA	12	V649I (GTC→ATC)	letB	lipophilic envelope spanning tunnel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

GGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGA  >  NC_000913/1919337‑1919635                                                                                                                      |                                                                                                                                                                                      ggTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGAGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGATTCACGCTTTCGATGACGGAAAACTGGCGATCGGGCTGCCAATTCGCTATCTCGGTATTGATAACGGGCAAATCCAg                                                                                                                                        >  1:144309/1‑165 (MQ=255)     ncAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCCCGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGGACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTAcg                                              >  1:21006/2‑251 (MQ=255)                                     aTTCTGTAGGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCggn              <  1:135805/251‑2 (MQ=255)                                            aTGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGc       <  1:9702/251‑1 (MQ=255)                                                          gCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCANTGANNNNNAg                                                                                                    >  2:130070/1‑144 (MQ=255)                                                          gCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTgn  <  1:130070/242‑2 (MQ=255)                                                                 gTGCGNCTGGCNGNNAGATTACGCTTCACGCTTTNGATGCCGGAAAACTGNCGATNGGGATNccn                                                                                                                                                                            >  2:85818/1‑64 (MQ=255)                                                                                 naTTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGa                                                                                                                                       >  1:132037/2‑86 (MQ=255)                                                                                 gATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGa                                                                                                                                       <  2:132037/86‑1 (MQ=255)                                                                                                    gATGCCGGAAAACTGGCGATCGGGATGCCAATTCGCTATCTCGGTTTTGATATCGGGCAAATCCAGACGCTGGTGCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAat                                                                           <  1:115871/127‑1 (MQ=255)                                                                                                                  ggCGATCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAg                                                                                                                                        <  1:110091/52‑1 (MQ=255)                                                                                                                  ggCGANCGNGNTGNNANTTCGNTATCTCGGTATTGATATCGGGCAAATCCAg                                                                                                                                        >  2:110091/1‑52 (MQ=255)                                                                                                                      |                                                                                                                                                                                      GGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCACGCTTTCGATGCCGGAAAACTGGCGGTCGGGATGCCAATTCGCTATCTCGGTATTGATATCGGGCAAATCCAGACGCTGGATCTGATTACCGCACGCAATGAAGTACAGGCAAAGGCGGTACTTTACCCGGAATATGTCCAGACCTTTGCTCGCGGTGGTACGCGCTTCTCAGTGGTCACACCGCAAATTTCGGCAGCTGGCGTTGA  >  NC_000913/1919337‑1919635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑