Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,919,097	G→A	G530R (GGA→AGA)	letB →	lipophilic envelope spanning tunnel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,919,097	0	G	A	87.5%	12.5 / NA	8	G530R (GGA→AGA)	letB	lipophilic envelope spanning tunnel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/4);  minor base C (1/0);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATG  >  NC_000913/1918873‑1919262                                                                                                                                                                                                                                 |                                                                                                                                                                      gCCGTGTCGATGTGAAAGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTTTGCCGGGCGATAAAGGGGAGATGAAAACCAGCTCTCCACTGTTTTCCAATTTTGAAAAAACGCTGGGGAACAGGCTTAGCGATTTACCCCCCCCCACCGTGGGTTTGAGTGCAGAGAAGCTGCCGGGTGTGCCGGCAAGATcagaacggaagagcacccgtctt                                                                                                                                             >  1:215171/1‑229 (MQ=255)                aGGTGGGGCTGTATTGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGATCAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGGTCAGAGACTCTGCCGGATGTGCAGGCAAGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTg                                                                                                                              <  1:145974/251‑1 (MQ=255)                          ggATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACGGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAAGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGt                                                                                                                      <  1:196640/249‑1 (MQ=255)                                                      gcCTCAGAATGGATCAATGGCGGGATACGTCTTCTGCTGGGCGATAAAGGTGAGATGAACGCCAGGTATCTCCTGTATGCCAATTTGGAAAAAGAGCTGGAGAACAGCCCTAGCGCTTTACCCACCACAACCGCGAGTTTGAGTGCAGAGCCGCTGCCGGATGTGCCGGCACGATCGGTAGTGCTGTACCGTAAATTTGAACTTGGGGGAGCGATTACCGTGCATCCGCGAGCCAACGCGTTTGATATCg                                                                                         >  1:189257/1‑250 (MQ=255)                                                                                    aTTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCTGATGTGCAGGCAAGATCGGTAGTGCTGTACCGTAAATTTGAAGTGGGTGAAGTGATTACCGTGCATCCGCGATCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAAcn                                                           <  1:25083/250‑2 (MQ=255)                                                                                                                             aCTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAAGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCATCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCg                  >  1:190558/1‑250 (MQ=255)                                                                                                                                            ggAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCAACCACAACCGTGAGTAGGCGTGCAGAGACGCTGCCGGATGTGCAGGCAAGATCGGTCGTGCTGTACCGTAAATTTTAAGATGGTGAAGAGATTACCGTGCATCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGCAAGTTCAGCtaaagg  >  1:64880/1‑246 (MQ=255)                                                                                                                                                                                                                  ccGGATGTGCAGGCAAGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCATTCGCGAGCTAACGCGTTTGAGATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAg                          <  1:178936/157‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                      GCCGTGTCGATGTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATG  >  NC_000913/1918873‑1919262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑