Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,069	G→A	P156S (CCT→TCT)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,069	0	G	A	100.0%	23.8 / NA	9	P156S (CCT→TCT)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/6);  total (3/6)

TTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGC  >  NC_000913/1914835‑1915303                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           ttCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTGTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGcc                                                                                                                                                                                                                             <  1:76627/250‑1 (MQ=255)                                                                   cGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCAACTTACGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGAATGCTGTTCTTCGCGAGGAGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGGCGGACGTGGCTTGCGTTCGCGGCGCGGGGCGTCATCTTTCTCAcc                                                                                                                                                           >  1:163698/1‑250 (MQ=255)                                                                                                     cgcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt                                                                                                                        >  1:51730/1‑251 (MQ=255)                                                                                                                     ttCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCGTTTGCGCACGAGATTTACGTTCATCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTTCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgt                                                                                                          <  1:123086/249‑1 (MQ=255)                                                                                                                        aCCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTGGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                                                                                     <  1:157363/251‑1 (MQ=255)                                                                                                                                                                            gCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTg                                                  <  1:73483/250‑1 (MQ=255)                                                                                                                                                                                                                   tctcTACCGGCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGac           <  1:62425/250‑1 (MQ=255)                                                                                                                                                                                                                           ggCTTTTGCGCACGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTgc  <  1:59981/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGAGATTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGGGCGTCTTCTTTCTCACCAGNNGTTGCgnn                                                                                                                                               >  2:12050/1‑96 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           TTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGC  >  NC_000913/1914835‑1915303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑