Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,913,816	G→A	T334I (ACC→ATC)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,913,816	0	G	A	100.0%	27.9 / NA	11	T334I (ACC→ATC)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

CTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCAC  >  NC_000913/1913706‑1914024                                                                                                               |                                                                                                                                                                                                                 cTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGCCTTTCTCTTTACCGACGGTCTTCACCGACATTTTACCCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCTGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatt                                                                                                                               >  1:218407/1‑194 (MQ=255)                                cAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGAc                                                        >  1:17426/1‑234 (MQ=255)                                    aCGCCGACTTTCTCTTTACCGACGGTCTTCACCGCCATTTTAACCGCGCGGTCTTCGAGACGCATAGGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACACGCAGGAAAAATTTCCAGACGAAc                                                                                                                                                        >  1:51125/1‑134 (MQ=255)                                    aCGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCNCGCGGTCTTNNAGNCGAAGNCNNNCNNNGANCAACGTTACAGTACGGGTCNTGNNCCCTTTNCCAGCAGGTAAAAGTTCCAGACGAAc                                                                                                                                                        <  2:51125/134‑1 (MQ=255)                                                        gACGGTCTTCACAGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCCATCACGTCAACCATCGGCTTGCCTGTTTGACCAaca                                                             >  1:51845/1‑205 (MQ=255)                                                           ggTCTTCACCGACATTTTAACCGCGCGGGCTGCGAGACGAATACGTTCACGGATTAACGTTACAGTACGGGTCGTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCGTCGTCGCTTTAAGTAAGGCAACCACATCAGCAAGACGCCAGCCAATCACGTCAACCAGCTGCTTTCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGac            <  1:126961/251‑1 (MQ=255)                                                                    cGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAAcc                                                                                                                      <  1:109990/136‑1 (MQ=255)                                                                    cGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTActc                         <  1:74406/229‑1 (MQ=255)                                                                     gcnaTTNTNNCNNCGCNCTCTTCGAGTCGAATACGTTCNNGGATCAACGNTACAGTACTGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTNACTGCCCTTNNGCNCTTTANTNNNGNNNACNACAGCAGCAAGACGCCAGCNAANNACGTCANCCATCGGCTTGCCTGTTTGACCAaca                                                             <  2:51845/189‑1 (MQ=255)                                                                     gACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACGCCGGCAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGccac  <  1:115325/251‑1 (MQ=255)                                                                                                       gTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGAAAAATTTCCAGACGAACTTTCCTGCCCTTCGGACCTTTAATTAAGGCAACCACATCCTCAAGACgcc                                                                                                      >  1:104049/1‑117 (MQ=255)                                                                                                               |                                                                                                                                                                                                                 CTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCAC  >  NC_000913/1913706‑1914024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑