Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,390	G→A	T399I (ACC→ATC)	yoaE ←	TerC family inner membrane protein YoaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,390	0	G	A	100.0%	38.5 / NA	15	T399I (ACC→ATC)	yoaE	TerC family inner membrane protein YoaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

CATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCG  >  NC_000913/1900205‑1900635                                                                                                                                                                                          |                                                                                                                                                                                                                                                      catcgGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGTCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGtt                                                                                                                                                                                      <  1:27388/251‑1 (MQ=255)                      cAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCgg                                                                                                                                                                  <  1:79183/250‑1 (MQ=255)                               nctgcAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGACGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCCAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGGTTCCGGGACGATAATCGCCGGAGACGCCGa                                                                                                                                                         >  1:20299/2‑250 (MQ=255)                                     aGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAAtn                                                                                                                                                  <  1:175975/251‑2 (MQ=255)                                                                 cagccagcCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTCGTACCACACCAAACTCGTTGATCACGATAACAAAGCGCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAgcg                                                                                                                      <  1:6518/251‑1 (MQ=255)                                                                       gccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGccacc                                                                                                                 >  1:48329/1‑250 (MQ=255)                                                                                               cGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTACCAGCACATCCAGCGGCTTGACCAGACCGTGTACCACACCAAACTCGTTGATCACGATGACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTGTCCGGGACGATAATCGCCGGAGACgn                                                                                                                                                            <  1:26457/183‑2 (MQ=255)                                                                                                taatagtgcaccgccgccGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATAc                                                                                        <  1:99744/234‑1 (MQ=255)                                                                                                            gCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTGGTACCACACCAAACTCGTTGATCACGATAACAAACCTCCCGCGAGCACGACGCAGCACGCCCAACATGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGTAAACGCCGAAGCAATCTCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATc                                                                           <  1:195501/251‑1 (MQ=255)                                                                                                                     aaTTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCTAGGGTTGCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCAcc                                                                  <  1:153295/251‑1 (MQ=255)                                                                                                                                   ngCTTCCAGCACATCCAGCGGCGTGAACAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCggg                                                     >  1:64570/2‑250 (MQ=255)                                                                                                                                         cAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAg                                                                                                                                                                                                                               >  1:59533/1‑74 (MQ=255)                                                                                                                                          aGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCACCGGAGACGCCGAAGCAATCGCCGCCTCCTCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCCCGTACAATACCGATGATTTCATCCCGTTCACCGCGACATACCGGGAACAGACt                                             >  1:95572/1‑251 (MQ=255)                                                                                                                                                     cggcgTGACCAGACCTTGTACCACACCAAACGCGTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCgn                                                                                                                                                                                 <  1:38231/108‑2 (MQ=255)                                                                                                                                                                                      gTTGATCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCAAAAAGGTTGATCGGACCGAGGGAATCCGGGACGCTAATCGCCGGAGACGCCGACGGAATCGCCGGCACATACACGACCTCTCCCAGCGCCAACAGAAGTTCATTAGAACGTACAATACCGATGATTTCATCAAGGTCACCGCGACATACCGGGAACAGACTGTGCGGCAAACAGAGCAGTTGCTCGCGGATTGCATCGACCCCg  >  1:213725/1‑250 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCG  >  NC_000913/1900205‑1900635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑